TRAF2 Recombinant Rabbit Monoclonal Antibody

货号:
AWA12681
应用:
IHC-P,IF-T,FCM
反应性:
Human,Mouse,Rat
来源:
Rabbit
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

     

    Host Species:

    Rabbit

    Reactivity:

    Human,Mouse,Rat

    Molecular Wt:

    56 KDa


    Clonality:

    Monoclonal 

    Isotype:

    IgG

    Concentration:

    1 mg/ml


    Other Names:

    TRAF2; TRAF2_HUMAN; TRAF 2; TRAP; TRAP3; TRAP 3; E3 ubiquitin-protein ligase TRAF2; TNF receptor associated factor 2; TNF receptor-associated factor 2; TNF receptor-associated protein; Tumor necrosis factor type 2 receptor associated protein 3

     


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    -20°C,1 year



    Applications

     

    IHC-P 1:50-1:200

    IF-T 1:50-1:200

    FCM 1:50-1:100

     



    Immunogen

    Information

    Gene Name:

    TRAF2

    Protein Name:

    TNF receptor-associated factor 2


    Gene ID:

    7186 (Human)     

    22030 (Mouse)     

    311786 (Rat)

     

    SwissPro:

    Q12933 (Human)     

    P39429 (Mouse)     

    B5DFH7 (Rat)

     


    Subcellular Location:

    Cytoplasm.


    Immunogen:

    Synthetic peptide within N-terminal human TRAF2.


    Specificity:

    TRAF2 Monoclonal Antibody detects endogenous levels of TRAF2 protein.


    Product images
    TRAF2 Recombinant Rabbit Monoclonal Antibody - 1 Fig: Fluorescence immunohistochemical analysis of lewis cell derived xenograft tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-TRAF2 antibody (AWA12681) at 1/200 dilution.

    The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0691). The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA12681) at 1/200 dilution for 2 hour at 37℃ or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (Purple). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.
    TRAF2 Recombinant Rabbit Monoclonal Antibody - 2 Fig: Fluorescence immunohistochemical analysis of Mouse-lung tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-TRAF2 antibody (AWA12681) at 1/200 dilution.

    The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0691). The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA12681) at 1/200 dilution for 2 hour at 37℃ or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (Purple). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.
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