HLA-DRA Recombinant Rabbit Monoclonal Antibody

货号：

AWA12675

应用：

WB,IHC-P,IF-C,IF-T,mIHC,FCM

反应性：

Human,Rat

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human, Rat

Molecular Wt:

Predicted MW: 29 kDa
Observed MW: 35 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1.113mg/ml

Other Names:

MHC class II antigen DR; HLA-DRA1; HLA DRA1; HLA-DR; HLA DRB; HLA-DRA

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

Applications

WB 1:1000-1:5000
IHC-P 1:100-1:1000
IF-C 1:100-1:800
IF-T 1:100-1:1000
mIHC 1:100-1:1000
FCM 1:50-1:200

Immunogen
Information

Gene Name:

HLA-DRA

Protein Name:

HLA class II histocompatibility antigen, DR alpha chain

Gene ID:

3122 (Human)

SwissPro:

P01903 (Human)

Immunogen
Information

Subcellular Location:

Cell membrane. Endoplasmic reticulum membrane. Early endosome membrane. Late endosome membrane. Lysosome membrane. Autolysosome membrane.

Immunogen:

Synthetic peptide within human HLA-DRA. AA range: 159-195.

Specificity:

HLA-DRA Monoclonal Antibody detects endogenous levels of HLA-DRA protein.

Product images
	Fig: Multiplex immunohistochemistry analysis of Rat-intestinal tissue (Formalin/PFA-fixed paraffin-embedded sections). Merged staining of anti-CD27 (AWA10960; green; TSA-520:AWI0688), anti-HLA-DRA (AWA12675; red; TSA-570: AWI0689), anti-EPCAM (AWA13608; white; TSA-690: AWI0691). Antibody Wash Solution (for mIHC) (AWI0707, 37℃, 20 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity. The section was incubated in three rounds of staining; in the order of CD27 (AWA10960) (1/200 dilution), anti-HLA-DRA (AWA12675) (1/200 dilution), anti-EPCAM (AWA13608) (1/200 dilution); each using a separate fluorescent tyramide signal amplification system. DAPI (blue, AWC0291) was used as a nuclear counter stain. Microscopy and pseudocoloring of individual TSA dyes was performed using a pannoramic midi.

	Fig: Immunocytochemistry analysis of Thp-1 cells labeling HLA-DRA with Rabbit anti-HLA-DRA antibody（AWA12675）at 1/250 dilution(Green ). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-HLA-DRA antibody （AWA12675）at 1/250 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, AWS0005) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).

	Fig : Immunohistochemical analysis of paraffin-embedded Rat-brain tissue with Rabbit anti-HLA-DRA antibody (AWA12675) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA12675) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig : Western blot analysis of HLA-DRA on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA12675, 1/2000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Raji cell Predicted band size: 29 kDa Observed band size: 35 kDa

	Fig: Fluorescence immunohistochemical analysis of Rat-Colon tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-HLA-DRA antibody (AWA12675) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0688). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 5% BSA for 60 minutes at 37℃, washed with ddH2O and PBS, and then probed with the primary antibody (AWA12675) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (green). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.

	Fig: Fluorescence immunohistochemical analysis of Rat-spleen tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-HLA-DRA antibody (AWA12675) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0688). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 5% BSA for 60 minutes at 37℃, washed with ddH2O and PBS, and then probed with the primary antibody (AWA12675) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (green). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.

	Fig : Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue with Rabbit anti-HLA-DRA (AWA12675) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA12675) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig : Immunohistochemical analysis of paraffin-embedded Rat-lung tissue with Rabbit anti-HLA-DRA (AWA12675) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA12675) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig : Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue with Rabbit anti-HLA-DRA antibody (AWA12675) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA12675) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig : Immunohistochemical analysis of paraffin-embedded Rat-liver tissue with Rabbit anti-HLA-DRA antibody (AWA12675) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA12675) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig : Immunohistochemical analysis of paraffin-embedded Rat-thymus tissue with Rabbit anti-HLA-DRA (AWA12675) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA12675) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig: Immunocytochemistry analysis of Hela cells labeling HLA-DRA with Rabbit anti-HLA-DRA antibody (AWA12675) at 1/200 dilution(Green). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-HLA-DRA antibody (AWA12675) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, AWS0005c) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).

	Overlay histogram showing THP-1 cells stained with HLA-DR (green line). The cells were fixed, permeabilized and stained with the primary antibody(AWA12675, 1µg/1x106 cells) for 30 min at 4°C. The secondary antibody used was an Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody (AWS0005) at 1/500 dilution for 30 min at 4ºC. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).