Ki67 Recombinant Rabbit Monoclonal Antibody

货号：

AWA10320

应用：

WB,IHC-P,IHC-F,IF-C,IF-T,mIHC,FCM

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

参考文献

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat

Molecular Wt:

Predicted MW: 359 kDa
Observed MW: 359 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1.065mg/ml

Other Names:

Antigen KI 67; Antigen KI-67; Antigen Ki67; Ki 67; KI67; KIA; MKI67; MIB; MIB 1; Antigen identified by monoclonal antibody Ki 67; Antigen identified by monoclonal antibody Ki-67; PPP1R105; Proliferation marker protein Ki-67; Proliferation related Ki 67 antigen

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

Applications

WB 1:500-1:2000
IHC-P 1:100-1:1000
IHC-F 1:100-1:1000
IF-C 1:50-1:200
IF-T 1:100-1:1000
mIHC 1:100-1:1000
FCM 1:500-1:1000

Immunogen
Information

Gene Name:

MKI67

Protein Name:

Proliferation marker protein Ki-67

Gene ID:

4288 (Human)
17345 (Mouse)
246042 (Rat)

SwissPro:

P46013 (Human)
E9PVX6 (Mouse)
Q5RJM0 (Rat)

Subcellular Location:

Chromosome. Nucleus. Nucleus, nucleolus.

Immunogen:

Synthetic peptide within human Ki67. AA range: 1040-1080.

Specificity:

Ki67 Monoclonal Antibody detects endogenous levels of Ki67 protein.

Product images
	Fig: Multiplex immunohistochemistry analysis of Mouse-spleen tissue (Formalin/PFA-fixed paraffin-embedded sections). Merged staining of anti-Ki67 (AWA10320; green; TSA-520:AWI0688), anti-AGMAT (AWA54578; red; TSA-570: AWI0689), anti-CD20 (AWA10037; white; TSA-690: AWI0691). Antibody Wash Solution (for mIHC) (AWI0707, 37℃, 20 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity. The section was incubated in three rounds of staining; in the order of Ki67 (AWA10320) (1/800 dilution), anti-AGMAT (AWA54578) (1/200 dilution), anti-CD20 (AWA10037) (1/200 dilution); each using a separate fluorescent tyramide signal amplification system. DAPI (blue, AWC0291) was used as a nuclear counter stain. Microscopy and pseudocoloring of individual TSA dyes was performed using a pannoramic midi. This image was generated from the hybridoma version.
	Fig : Western blot analysis of Ki67 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA10320, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: A431 cell Lane 2: A549 cell Lane 3: SIHa cell Lane 4: HepG2 cell Lane 5: CAL27 cell Lane 6: MCF-7 cell Lane 7: Raji cell Lane 8: HaCaT cell Lane 9: PANC-1 cell Predicted molecular weight:359 kDa Observed molecular weight:359 kDa
	Fig : Western blot analysis of Ki67 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA10320, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SIHa cell Lane 2: EL-4-B5 cell Predicted molecular weight:359 kDa Observed molecular weight:359 kDa
	Fig: Immunocytochemistry analysis of U2SO cells labeling Ki67 with Rabbit anti-Ki67 antibody (AWA10320) at 1/50 dilution(green). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-Ki67 antibody (AWA10320) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™488, AWS0005) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).
	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-large intestine tissue with Rabbit anti-Ki67 antibody (AWA10320) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10320) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue with Rabbit anti-KI67 (AWA10320) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10320) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-heart tissue with Rabbit anti-KI67 (AWA10320) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10320) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded rat-testicle tissue with Rabbit anti-Ki67 (AWA10320) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10320) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded rat-spleen tissue with Rabbit anti-Ki67 (AWA10320) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10320) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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