xCT/SLC7A11 Recombinant Rabbit Monoclonal Antibody
-
-
- 20μL
- ¥620
- 有库存
-
- 50μL
- ¥1250
- 有库存
-
- 100μL
- ¥2200
- 有库存
Product Details
| Host Species: Rabbit | Reactivity: Human,Mouse,Rat | Molecular Wt: 55 kDa | |
Clonality: Monoclonal | Isotype: IgG | Concentration: 1 mg/ml | ||
Other Names: CCBR1; Cysteine/glutamate transporter; SLC7A11; solute carrier family 7; xCT | ||||
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. | ||||
Purification: Affinity-chromatography | ||||
Storage: -20°C,1 year | ||||
Applications
| WB 1:1000 IP 1-2μg/sample IHC 1:100-1:400 | |||
Immunogen Information | Gene Name: SLC7A11 | Protein Name: Cystine/glutamate transporter | ||
Gene ID: 23657 (Human) 26570 (Mouse) 310392 (Rat)
| SwissPro: Q9UPY5 (Human) Q9WTR6 (Mouse)
| |||
Immunogen: Synthetic peptide within Human xCT. AA range:1-112. | ||||
Specificity: xCT Monoclonal Antibody detects endogenous levels of xCT protein. |
Product images | |
Fig : Western blot analysis of xCT/SLC7A11 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA10085, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HEPG2 cell Lane 2:SW620 cell Lane 3: A549 cell Lane 4: HEPA1-6 cell Lane 5: AML-12 cell Lane 6: HCT116 cell Lane 7: MC-38 cell Lane 8: PC-12 cell Lane 9: NPK-49F cell Lane 10: RBL-2H3 cell Calculated molecular weight:55 kDa Observed molecular weight:55 kDa, 35-40 kDa |
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Fig : Immunohistochemical analysis of paraffin-embedded Mouse-small intestine tissue with Rabbit anti-xCT-SLC7A11 (AWA10085) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10085) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig : Immunohistochemical analysis of paraffin-embedded Mouse-spleen tissue with Rabbit anti-xCT-SLC7A11 (AWA10085) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10085) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
-
-
- 20μL
- ¥620
- 1-3个工作日
-
- 50μL
- ¥1250
- 1-3个工作日
-
- 100μL
- ¥2200
- 1-3个工作日
-
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