STAT3(Phospho Ser727) Recombinant Rabbit Monoclonal Antibody

货号：

AWA12673

应用：

WB,IHC-P,IF-C,IF-T,IP

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ￥620 1-3个工作日
50μL ￥1250 1-3个工作日
100μL ￥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat

Molecular Wt:

88 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

STAT3; STAT 3; APRF; Signal transducer and activator of transcription 3; Acute-phase response factor; HIES; ADMIO; ADMIO1; Phospho-STAT3-S727

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:500-1:2000

IHC-P 1:50-1:200

IF-C 1:50-1:200

IF-T 1:50-1:200

IP Use at an assay dependent concentration.

Immunogen

Information

Gene Name：

STAT3

Protein Name：

Signal transducer and activator of transcription 3

Gene ID：

6774 (Human)

20848 (Mouse)

25125 (Rat)

SwissPro：

P40763 (Human)

P42227 (Mouse)

P52631 (Rat)

Subcellular Location：

Cytoplasm. Nucleus.

Immunogen：

Synthetic phospho peptide corresponding to residues surrounding Ser727 of human STAT3. AA range: 701-750.

Specificity:

Phospho STAT3 (Ser727) Monoclonal Antibody detects endogenous levels of Phospho STAT3 (Ser727) protein.

Product images
	Fig : Western blot analysis of STAT3(S727) on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 90 minutes at room temperature. The primary antibody (AWA12673, 1/1000) was used in TBST at room temperature for 90 minutes. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: MCF-7 cell Lane 2: NIH3T3 cell Lane 3: K562 cell Lane 4: HeLa cell
	Fig : Western blot analysis of STAT3 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA12673, 1/1000) was used in PBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: U251 cell Lane 2: Jurkat cell Lane 3: HEK-293 cell
	Fig: Immunocytochemistry analysis of Hela cells labeling STAT3(Phospho Ser727) with Rabbit anti-STAT3(Phospho Ser727) antibody (AWA12673) at 1/50 dilution (Green). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-STAT3(Phospho Ser727) antibody (AWA12673) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, AWS0005c) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).
	Fig: Immunocytochemistry analysis of Hela cells labeling STAT3(Phospho Ser727) with Rabbit anti-STAT3(Phospho Ser727) antibody (AWA12673) at 1/50 dilution (green). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-STAT3(Phospho Ser727) antibody (AWA12673) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, AWS0005) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-testis tissue with Rabbit anti-STAT3(phospho-S727) antibody (AWA12673) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA12673) at 1/200 dilution for 2 hour at 37℃ or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-ovary tissue with Rabbit anti-STAT3(Phospho Ser727) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA12673) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue with Rabbit anti-STAT3(Phospho Ser727) (AWA12673) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA12673) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig: Fluorescence immunohistochemical analysis of Mouse-heart Tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-STAT3(Phospho Ser727) antibody (AWA12673) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 5% BSA for 60 minutes at 37℃, washed with ddH2O and PBS, and then probed with the primary antibody (AWA12673) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (green). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.
	Fig: Fluorescence immunohistochemical analysis of Rat-brain Tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-STAT3(Phospho Ser727) antibody (AWA12673) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 5% BSA for 60 minutes at 37℃, washed with ddH2O and PBS, and then probed with the primary antibody (AWA12673) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (green). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.
	Fig: Fluorescence immunohistochemical analysis of Mouse-bladder Tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-STAT3(Phospho Ser727) antibody (AWA12673) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 5% BSA for 60 minutes at 37℃, washed with ddH2O and PBS, and then probed with the primary antibody (AWA12673) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (green). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.
	Fig: Fluorescence immunohistochemical analysis of Rat-Liver Tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-STAT3(Phospho Ser727) antibody (AWA12673) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 5% BSA for 60 minutes at 37℃, washed with ddH2O and PBS, and then probed with the primary antibody (AWA12673) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (green). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.