4EBP1 Recombinant Rabbit Monoclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA12623

应用：

WB,IHC-P,IF-C,IF-T,FCM

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat

Molecular Wt:

13 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

4E BP1; 4E-BP1; 4EBP1; BP 1; BP-1; eIF4E binding protein 1; EIF4EBP1; PHAS IMGC4316

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:1000-1:2000

IHC-P 1:50-1:200

IF-C 1:50-1:200

IF-T 1:50-1:200

FCM 1:50-1:100

Immunogen

Information

Gene Name：

EIF4EBP1

Protein Name：

Eukaryotic translation initiation factor 4E-binding protein 1

Gene ID：

1978 (Human)

13685 (Mouse)

116636 (Rat)

SwissPro：

Q13541 (Human)

Q60876 (Mouse)

Q62622 (Rat)

Subcellular Location：

Cytoplasm. Nucleus.

Immunogen：

Synthetic peptide with Human 4EBP1. AA range: 1-49.

Specificity:

4EBP1 Monoclonal Antibody detects endogenous levels of 4EBP1 protein.

Product images
	Fig : Western blot analysis of 4EBP1 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA12623, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell Lane 2: HepA1-6 cell Lane 3: AML12 cell Lane 4: HSC-T6 cell Lane 5: LOVO cell Lane 6: RBL-21H3 cell Predicted molecular weight:13 kDa Observed molecular weight:13 kDa
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-Colon tissue with Rabbit anti-4EBP1 antibody (AWA12623) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA12623) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-Spleen tissue with Rabbit anti-4EBP1 antibody (AWA12623) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA12623) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.