AMPKα1 Recombinant Rabbit Monoclonal Antibody
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-
- 20μL
- ¥620
- 1-3个工作日
-
- 50μL
- ¥1250
- 1-3个工作日
-
- 100μL
- ¥2200
- 1-3个工作日
Product Details
| Host Species: Rabbit | Reactivity: Human,Mouse,Rat,Monkey | Molecular Wt: 64 kDa | |
Clonality: Monoclonal | Isotype: IgG | Concentration: 1 mg/ml | ||
Other Names: AMPK; ACACA kinase; AMPK alpha; AMPK Alpha 1; AMPK alpha 1 chain; AMPK subunit alpha 1; AMPK1; AMPK 1; AMPKa1; PRKAA1; PRKAA 1; AMPKalpha1; cb116; SNF1-like protein AMPK; SNF1A; Tau protein kinase PRKAA1
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Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. | ||||
Purification: Affinity-chromatography | ||||
Storage: -20°C,1 year | ||||
Applications
| WB 1:1000-1:5000 IHC-P 1:200-1:1000 IHC-F 1:100 IF-C 1:100-1:250 IF-T 1:50-1:200 FCM 1:500-1:1000 IP Use at an assay dependent concentration.
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Immunogen Information | Gene Name: PRKAA1 | Protein Name: 5'-AMP-activated protein kinase catalytic subunit alpha-1
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Gene ID: 5562 (Human) 105787 (Mouse) 65248 (Rat)
| SwissPro: Q13131 (Human) Q5EG47 (Mouse) P54645 (Rat)
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Subcellular Location: Cytoplasm. Nucleus. | ||||
Immunogen: Synthetic peptide within Human AMPKα1. AA range: 501-550. | ||||
Specificity: AMPKα1 Monoclonal Antibody detects endogenous levels of AMPKα1 protein. |
Product images | |
Fig : Western blot analysis of AMPKα1 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody ( AWA11932, 1/1000) was used in TBST(0.3%TWEEN20) at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Jurkat cell lysate Lane 2: K562 cell lysate Lane 3: Hela cell lysate Lane 4: HepG2 cell lysate Lane 5: MCF-7 cell lysate Lane 6: HEK293 cell lysate Lane 7: NRK-49F cell lysate Lane 8: COS7 cell lysate Lane 9: L929 cell lysate Lane 10: SW480 cell lysate Lane 11: C2C12 cell lysate Predicted molecular weight: 64KD Observed molecular weight: 65 KD Exposure time: 15 sceonds |
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Fig : Western blot analysis of AMPKα1 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody ( AWA11932, 1/1000) was used in TBST(0.3%Tween 20) at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: RBL-2H3 cell lysate Lane 2: SHZ-88 cell lysate Lane 3: PC12 cell lysate Lane 4: NIH/3T3 cell lysate Predicted molecular weight: 64KD Observed molecular weight: 65 KD Exposure time: 45 sceonds |
-
-
- 20μL
- ¥620
- 1-3个工作日
-
- 50μL
- ¥1250
- 1-3个工作日
-
- 100μL
- ¥2200
- 1-3个工作日
-
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