ATG4B Recombinant Rabbit Monoclonal Antibody

货号:
AWA11356
应用:
WB
反应性:
Human,Mouse,Rat,Monkey
来源:
Rabbit
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

     

    Host Species:

    Rabbit

    Reactivity:

    Human,Mouse,Rat,Monkey

    Molecular Wt:

    44 kDa


    Clonality:

    Monoclonal

    Isotype:

    IgG

    Concentration:

    1 mg/ml


    Other Names:

    APG4B; ATG4B; AUTL1; Autophagin 1; Autophagin-1; Cysteine protease ATG4B; hAPG4B; KIAA0943; APG4 autophagy 4 homolog B; AUTL1; MGC1353

     


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    -20°C,1 year



    Applications

     

    WB 1:1000



    Immunogen

    Information

    Gene Name:

    ATG4B

    Protein Name:

    Cysteine protease ATG4B


    Gene ID:

    23192 (Human)     

    66615 (Mouse)     

    316640 (Rat)

    SwissPro:

    Q9Y4P1 (Human)     

    Q8BGE6 (Mouse)     

    Q62625  (Rat)

     


    Subcellular Location:

    Cytoplasm. Cytoplasm, cytosol. Cytoplasmic vesicle, autophagosome. Endoplasmic reticulum. Mitochondrion.

     


    Immunogen:

    Recombinant protein within Human ATG4B. AA range: 221-320.


    Specificity:

    ATG4B Monoclonal Antibody detects endogenous levels of ATG4B protein.




    Product images
    ATG4B Recombinant Rabbit Monoclonal Antibody - 1 Fig : Western blot analysis of ATG4B on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA11356, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: U87-MG cell
    Lane 2: MCF-7 cell
    Lane 3: K562 cell
    Lane 4: COS7 cell
    Lane 5: Rat Brain
    Lane 6: Hek293 cell
    Lane 7: MC-38 cell
    Lane 8: Jurkat cell
    Lane 9: C2CL2 cell


    Exposure time: 15 seconds
    Predicted band size: 44 kDa
    Observed band size: 48 kDa
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