CCR7 Recombinant Rabbit Monoclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA11341

应用：

WB,IHC-P,IF-C,IF-T,IP

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat

Molecular Wt:

43 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

BLR2; BLR 2; C C chemokine receptor type 7; C-C chemokine receptor type 7; C C CKR 7; C C CKR 7; CC CKR 7; C-C CKR-7; CC chemokine receptor 7; CC chemokine receptor type 7; CCCKR7; CCR 7; CCR-7; CCR7; CCR7_HUMAN; CD197; CD 197; CD197 antigen; CDw197; CMKBR7; EBI 1; EBI1; Ebi1h; EVI1; EVI 1; MIP 3 beta receptor; MIP-3 beta receptor; MIP3 Beta Receptor

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:1000-1:5000

IHC-P 1:200

IF-C 1:200-1:500

IF-T 1:200

IP 1-2μg/sample

Immunogen

Information

Gene Name：

CCR7

Protein Name：

C-C chemokine receptor type 7

Gene ID：

1236 (Human)

12775 (Mouse)

287673 (Rat)

SwissPro：

P32248 (Human)

P47774 (Mouse)

Q6U2D6 (Rat)

Subcellular Location：

Cell membrane.

Immunogen：

Synthetic peptide within Human CCR7. AA range: 13-62.

Specificity:

CCR7 Monoclonal Antibody detects endogenous levels of CCR7 protein.

Product images
	Fig : Western blot analysis of CCR7 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA11341, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell Lane 2: Jurkat cell Lane 3: MCF-7 cell Lane 4: K562 cell Lane 5: HL-60 cell Lane 6: EL-4-B5 cell Lane 7: Rat brain tissue Lane 8: PC12 cell Predicted molecular weight:43KD Observed molecular weight:40-43KD Exposure time:7 sec
	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-spleen tissue with Rabbit anti-CCR7 antibody (AWA11341) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11341) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig: Fluorescence immunohistochemical analysis of Lewis cell derived xenograft tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-CCR7 antibody (AWA11341) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0691). The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11341) at 1/200 dilution for 2 hour at 37℃ or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (Purple). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.
	Fig: Fluorescence immunohistochemical analysis of RM-1 cell derived xenograft tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-CCR7 antibody (AWA11341) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0691). The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11341) at 1/200 dilution for 2 hour at 37℃ or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (Purple). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.