AQP1 Recombinant Rabbit Monoclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA11338

应用：

WB,IHC-P,IF-C,IF-T,mIHC

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat

Molecular Wt:

28 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

AQP 1; AQP CHIP; AQP-1; AQP1; AQP1_HUMAN; Aquaporin1; Aquaporin 1; Aquaporin-1; Aquaporin CHIP; Aquaporin-CHIP; CHIP28; CHIP 28; CO; Urine water channel; Colton blood group; MGC26324; Water channel protein CHIP 29

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:2000-1:5000

IHC-P 1:200-1:1000

IF-C 1:50-1:200

IF-T 1:50-1:200

mIHC 1:5000

Immunogen

Information

Gene Name：

AQP1

Protein Name：

Aquaporin-1

Gene ID：

358 (Human)

11826 (Mouse)

25240 (Rat)

SwissPro：

P29972 (Human)

Q02013 (Mouse)

P29975 (Rat)

Subcellular Location：

Cell membrane.

Immunogen：

Synthetic peptide within Human AQP1. AA range: 245-269.

Specificity:

AQP1 Monoclonal Antibody detects endogenous levels of AQP1 protein.

Product images
	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue with Rabbit anti-AQP1 antibody (AWA11338) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11338) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Western blot analysis of AQP1 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA11338, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: PC12 cell Lane 2: NRK-49F cell Predicted molecular weight:29 kDa Observed molecular weight:29 kDa（The bands greater than 30 KD may be glycosylated AQP1） Exposure time:45 sec
	Fig: Fluorescence immunohistochemical analysis of Mouse-kidney tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-AQP1 antibody (AWA11338) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0691). The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11338) at 1/200 dilution for 2 hour at 37℃ or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (Purple). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.
	Fig: Fluorescence immunohistochemical analysis of Rat-kidney tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-AQP1 antibody (AWA11338) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0691). The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11338) at 1/200 dilution for 2 hour at 37℃ or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (Purple). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.