CD40L Recombinant Rabbit Monoclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA11337

应用：

WB,IHC-P,IF-C,IF-T,FCM

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat

Molecular Wt:

29 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

CD154; CD40 L; CD40 ligand; CD40L; CD40L/CD154; CD40LG; gp39; hCD40L; HIGM1; IGM; IMD3; T BAM; T cell antigen Gp39; TNF related activation protein;TNFSF5; TRAP

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:1000-1:5000

IHC-P 1:50-1:200

IF-C 1:50-1:200

IF-T 1:50-1:200

FCM 1:50-1:100

Immunogen

Information

Gene Name：

CD40LG

Protein Name：

CD40 ligand

Gene ID：

959 (Human)

21947 (Mouse)

84349 (Rat)

SwissPro：

P29965 (Human)

P27548 (Mouse)

Q9Z2V2 (Rat)

Subcellular Location：

Cell membrane. Cell surface. Secreted.

Immunogen：

Synthetic peptide within Human CD40L. AA range: 103-150.

Specificity:

CD40L Monoclonal Antibody detects endogenous levels of CD40L protein.

Product images
	Fig : Western blot analysis of CD40L on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody ( AWA11337, 1/1000) was used in PBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: K562 cell Lane 2: 3D4/21 cell Lane 3: EL-4-B5 cell Lane 4: SHZ-88 cell Lane 5: 4T1 cell Lane 6: NRK-49F cell Predicted molecular weight: 29.3kDa Observed molecular weight: 37 kDa Exposure time: 90 seconds
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue with Rabbit anti-CD40L antibody (AWA11337) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11337) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Western blot analysis of CD40L on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody ( AWA11337, 1/1000) was used in PBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: RAW264.7 cell Lane 2: Hela cell Lane 3: PC12 cell Lane 4: HEK293 cell Predicted molecular weight: 29.3kDa Observed molecular weight: 37 kDa Exposure time: 7 minutes