CHAT Recombinant Rabbit Monoclonal Antibody

货号:
AWA11332
应用:
WB,IHC-P,IHC-F,IF-C,IF-T,FCM,IP
反应性:
Human,Mouse,Rat
来源:
Rabbit
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

     

    Host Species:

    Rabbit

    Reactivity:

    Human,Mouse,Rat

    Molecular Wt:

    83 kDa


    Clonality:

    Monoclonal 

    Isotype:

    IgG

    Concentration:

    1 mg/ml


    Other Names:

    CHAT; ChAT; CHOACTase; Choline acetylase; choline acetyltransferase; Choline Acetyltransferase; Choline O acetyltransferase; Choline O-acetyltransferase; Acetyl CoA choline O acetyltransferase; CMS1A; CLAT_HUMAN; CMS1A2; EC 2.3.1.6; OTTHUMP00000019583; OTTHUMP00000019584

     


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    -20°C,1 year



    Applications

     

    WB 1:5000

    IHC-P 1:50-1:200

    IHC-F 1:100

    IF-C 1:50-1:200

    IF-T 1:50-1:200

    FCM 1:50-1:100

    IP Use at an assay dependent concentration.

     



    Immunogen

    Information

    Gene Name:

    CHAT

    Protein Name:

    Choline O-acetyltransferase


    Gene ID:

    1103 (Human)     

    12647 (Mouse)     

    290567 (Rat)

     

    SwissPro:

    P28329 (Human)     

    Q03059 (Mouse)     

    P32738 (Rat)

     


    Subcellular Location:

    Cytoplasm. Cytosol. Neuron projection. Nucleus. Synapse.


    Immunogen:

    Synthetic peptide within Human CHAT. AA range: 699-748.


    Specificity:

    CHAT Monoclonal Antibody detects endogenous levels of CHAT protein.


    Product images
    CHAT Recombinant Rabbit Monoclonal Antibody - 1 Fig : Western blot analysis of CHAT on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA11332, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: U251 cell
    Lane 2: SH-SY5Y cell
    Lane 3: GL261 cell
    Lane 4: Rat brain tissue

    Predicted molecular weight:83 kDa
    Observed molecular weight:83 kDa
    CHAT Recombinant Rabbit Monoclonal Antibody - 2 Fig : Immunohistochemical analysis of paraffin-embedded Rat-stomach tissue with Rabbit anti-CHAT antibody (AWA11332) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11332) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
    CHAT Recombinant Rabbit Monoclonal Antibody - 3 Fig : Immunohistochemical analysis of paraffin-embedded Mouse-brain tissue with Rabbit anti-CHAT (AWA11332) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11332) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
    CHAT Recombinant Rabbit Monoclonal Antibody - 4 Fig : Immunohistochemical analysis of paraffin-embedded Mouse-brain tissue with Rabbit anti-CHAT antibody (AWA11332) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11332) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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