ERK1/2 (Phospho Thr202/T185) Recombinant Rabbit Monoclonal Antibody

货号：

AWA11329

应用：

WB,IHC-P,IF-C,IF-T,FCM

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat

Molecular Wt:

43/41 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

ERK; ERK 1; ERK 2; ERK1; ERK1/2; ERK2; ERT2; HS44KDAP; HUMKER1A; MAP kinase 1; MAP kinase 3; MAP kinase isoform p44; MAPK 1; MAPK 2; MAPK 3; MAPK3; p44 ERK1; p44 MAPK; P44ERK1; P44MAPK; PRKM3

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:1000

IHC-P 1:200-1:1000

IF-C 1:100-1:500

IF-T 1:50-1:200

FCM 1:50-1:100

Immunogen

Information

Gene Name：

MAPK3/MAPK1

Protein Name：

Mitogen-activated protein kinase 3/Mitogen-activated protein kinase 1

Gene ID：

5595/5594 (Human)

26417/26413 (Mouse)

50689/116590 (Rat)

SwissPro：

P27361/P28482 (Human)

Q63844/P63085 (Mouse)

P21708/P63086 (Rat)

Subcellular Location：

Cytoplasm. Nucleus.

Immunogen：

Synthetic phospho peptide corresponding to residues surrounding Thr185 of Human Erk2.

Specificity:

Phospho ERK1/2 (Thr202/T185) Monoclonal Antibody detects endogenous levels of Phospho ERK1/2 (Thr202/T185) protein.

Product images
	Fig : Western blot analysis of ERK1/2(Phospho T202/T185) on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA11329, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SHZ-88 cell Lane 2: HepA1-6 cell Lane 3: CFSC-8B cell Predicted molecular weight:42/44KD Observed molecular weight:42/44KD Exposure time：45 sec
	Fig: Fluorescence immunohistochemical analysis of Mouse-duodenum tissue (Formalin/PFA-fixed paraffin-embedded sections). with rabbit anti-ERK1-2(Phospho Thr202 T185) antibody (AWA11329) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0691). The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11329) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (Purple). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.
	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-bladder tissue with Rabbit anti-EPK1/2 (AWA11329) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11329) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue with Rabbit anti-EPK1/2 (AWA11329) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11329) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig: Immunocytochemistry analysis of HELA cells labeling ERK1/2(Phospho Thr202/T185) with Rabbit anti-ERK1/2(Phospho Thr202/T185) antibody (AWA11329) at 1/50 dilution(Green). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-ERK1/2(Phospho Thr202/T185) antibody (AWA11329) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™488, AWS0005) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).