DNMT1 Recombinant Rabbit Monoclonal Antibody

货号:
AWA11325
应用:
WB,IHC-P,IF-C,IF-T
反应性:
Human,Mouse,Rat
来源:
Rabbit
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

     

    Host Species:

    Rabbit

    Reactivity:

    Human,Mouse,Rat

    Molecular Wt:

    183 kDa


    Clonality:

    Monoclonal

    Isotype:

    IgG

    Concentration:

    1 mg/ml


    Other Names:

    AIM; CXXC9; DNA methyltransferase HsaI; DNA MTase HsaI; DNMT; DNMT1; FLJ16293; M.HsaI; MCMT

     


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    -20°C,1 year



    Applications

     

    WB 1:500-1:2000

    IHC-P 1:50-1:1000

    IF-C 1:50-1:200

    IF-T 1:50-1:200

     



    Immunogen

    Information

    Gene Name:

    DNMT1

    Protein Name:

    DNA (cytosine-5)-methyltransferase 1


    Gene ID:

    1786 (Human)     

    13433 (Mouse)     

    84350 (Rat)

     

    SwissPro:

    P26358 (Human)     

    P13864 (Mouse)     

    Q9Z330 (Rat)

     


    Subcellular Location:

    Nucleus.


    Immunogen:

    Synthetic peptide within human DNMT1. AA range: 1506-1549. 


    Specificity:

    DNMT1 Monoclonal Antibody detects endogenous levels of DNMT1 protein.


    Product images
    DNMT1 Recombinant Rabbit Monoclonal Antibody - 1 Fig : Western blot analysis of DNMT1 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA11325, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: RSC96 cell
    Lane 2: MCF-7 cell
    Lane 3: 4T1 cell
    Lane 4: Hela cell
    Lane 5: CT26 cell
    Lane 6: HSC-T6 cell
    Lane 7: Raji cell
    Lane 8: EL-4-B5 cell

    Predicted molecular weight:183 kDa
    Observed molecular weight:183 kDa

    Exposure time:45 sec
    在线客服
    关闭
    小薇 薇薇 小艾 小奥
    联系销售
    技术支持

    image.png

    月琴艾碧维二维码.jpg