IκB-α(Phospho S32) Recombinant Rabbit Monoclonal Antibody
-
-
- 20μL
- ¥620
- 1-3个工作日
-
- 50μL
- ¥1250
- 1-3个工作日
-
- 100μL
- ¥2200
- 1-3个工作日
Product Details
| Host Species: Rabbit | Reactivity: Human,Mouse | Molecular Wt: 36 kDa | |
Clonality: Monoclonal | Isotype: IgG | Concentration: 1 mg/ml | ||
Other Names: I kappa B alpha; I-kappa-B-alpha; IkappaBalpha; IkB alpha; IKBA; Ikβ-α; MAD 3; MAD3; NF kappa B inhibitor alpha; NFKBI; NFKBIA
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Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. | ||||
Purification: Affinity-chromatography | ||||
Storage: -20°C,1 year | ||||
Applications
| WB 1:1000 IF 1:50-1:200 IP Use at an assay dependent concentration.
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Immunogen Information | Gene Name: NFKBIA | Protein Name: NF-kappa-B inhibitor alpha | ||
Gene ID: 4792 (Human) 18035 (Mouse)
| SwissPro: P25963 (Human) Q9Z1E3 (Mouse)
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Subcellular Location: Cytoplasm. Nucleus. | ||||
Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser32 of human IKB alpha.
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Specificity: Phospho IκB-α (S32) Monoclonal Antibody detects endogenous levels of Phospho IκB-α (S32) protein. |
Product images | |
Fig : Western blot analysis of IκB-α(Phospho Ser32) on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA11324, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SHZ-88 cell Lane 2: Hela cell treated with 20ng/ml TNF-αfor 5min Lane 3: MC38 cell Lane 4: HepA1-6 cell Lane 5: CFSC-8B cell Lane 6: Raji cell Lane 7: DU145 cell Lane 8: PANC-1 cell Predicted molecular weight:36 kDa Observed molecular weight:~40 kDa Exposure time:90 sec |
-
-
- 20μL
- ¥620
- 1-3个工作日
-
- 50μL
- ¥1250
- 1-3个工作日
-
- 100μL
- ¥2200
- 1-3个工作日
-
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