IκB-α(Phospho S32) Recombinant Rabbit Monoclonal Antibody

货号:
AWA11324
应用:
WB,IF,IP
反应性:
Human,Mouse
来源:
Rabbit
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

     

    Host Species:

    Rabbit

    Reactivity:

    Human,Mouse

    Molecular Wt:

    36 kDa


    Clonality:

    Monoclonal

    Isotype:

    IgG 

    Concentration:

    1 mg/ml


    Other Names:

    I kappa B alpha; I-kappa-B-alpha; IkappaBalpha; IkB alpha; IKBA; Ikβ-α; MAD 3; MAD3; NF kappa B inhibitor alpha; NFKBI; NFKBIA

     


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    -20°C,1 year



    Applications

     

    WB 1:1000

    IF 1:50-1:200

    IP Use at an assay dependent concentration.

     



    Immunogen

    Information

    Gene Name:

    NFKBIA

    Protein Name:

    NF-kappa-B inhibitor alpha


    Gene ID:

    4792 (Human)     

    18035 (Mouse)     

     

    SwissPro:

    P25963 (Human)     

    Q9Z1E3 (Mouse)     

     


    Subcellular Location:

    Cytoplasm. Nucleus.


    Immunogen:

    Synthetic phospho-peptide corresponding to residues surrounding Ser32 of human IKB alpha.

     


    Specificity:

    Phospho IκB-α (S32) Monoclonal Antibody detects endogenous levels of Phospho IκB-α (S32) protein.



    Product images
    IκB-α(Phospho S32) Recombinant Rabbit Monoclonal Antibody - 1 Fig : Western blot analysis of IκB-α(Phospho Ser32) on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA11324, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: SHZ-88 cell
    Lane 2: Hela cell treated with 20ng/ml TNF-αfor 5min
    Lane 3: MC38 cell
    Lane 4: HepA1-6 cell
    Lane 5: CFSC-8B cell
    Lane 6: Raji cell
    Lane 7: DU145 cell
    Lane 8: PANC-1 cell

    Predicted molecular weight:36 kDa
    Observed molecular weight:~40 kDa

    Exposure time:90 sec
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