CDK2 Recombinant Rabbit Monoclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA11322

应用：

WB,IHC-P,IF-C,IF-T,FCM,IP

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat

Molecular Wt:

34 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

CDK2; CDK2_HUMAN; CDKN2; Cyclin-dependent kinase 2; Cell division protein kinase 2; p33 protein kinase; p33(CDK2)

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:500-1:5000

IHC-P 1:50-1:200

IF-C 1:50-1:200

IF-T 1:50-1:200

FCM 1:1000

IP Use at an assay dependent concentration.

Immunogen

Information

Gene Name：

CDK2

Protein Name：

Cyclin-dependent kinase 2

Gene ID：

1017 (Human)

12566 (Mouse)

362817 (Rat)

SwissPro：

P24941 (Human)

P97377 (Mouse)

Q63699 (Rat)

Subcellular Location：

Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Nucleus, Cajal body. Cytoplasm. Endosome.

Immunogen：

Synthetic peptide within Human CDK2. AA range: 249-298.

Specificity:

CDK2 Monoclonal Antibody detects endogenous levels of CDK2 protein.

Product images
	Fig : Western blot analysis of CDK2 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA11322, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HEK293 cell Lane 2: Jurkat cell Lane 3: PC12 cell Lane 4: NRK-49F cell Lane 5: RBL-2H3 cell Lane 6: RAW264.7 cell Lane 7: THP-1 cell Lane 8: SIHa cell Lane 9: Hela cell Lane 10: LLC cell Predicted molecular weight:34KD Observed molecular weight:~30KD
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-Testicles tissue with Rabbit anti-CDK2 antibody (AWA11322) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11322) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig: Immunocytochemistry analysis of U2OS cells labeling CDK2 with Rabbit anti-CDK2 antibody (AWA11322) at 1/50 dilution(Red ). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-CDK2 antibody (AWA11322) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, AWS0006) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).