Cyclin E1 Recombinant Rabbit Monoclonal Antibody

货号:
AWA11321
应用:
IHC-P,IF-C,IF-T
反应性:
Human,Mouse,Rat
来源:
Rabbit
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

    Host Species:

    Rabbit

    Reactivity:

    Human,Mouse,Rat

    Molecular Wt:

    47 kDa


    Clonality:

    Monoclonal

    Isotype:

    IgG

    Concentration:

    1mg/ml


    Other Names:

    CCNE; CCNE1; Ccne1; cyclin E1; Cyclin Es; Cyclin Et; CyclinE; G1/S specific cyclin E1; G1/S specific cyclin E


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.



    Applications

    IHC-P 1:200-1000
    IF-C 1:50-1:200
    IF-T 1:100-1:500



    Immunogen

    Information

    Gene Name:

    CCNE1

    Protein Name:

    G1/S-specific cyclin-E1


    Gene ID:

    898 (Human)
    12447 (Mouse)
    25729 (Rat)

    SwissPro:

    P24864 (Human)
    Q61457 (Mouse)
    B1WC54 (Rat)


    Subcellular Location:

    Nucleus.


    Immunogen:

    Synthetic peptide within human Cyclin E1. AA range: 361-410.


    Specificity:

    Cyclin E1 Monoclonal Antibody detects endogenous levels of Cyclin E1 protein.




    Product images
    Cyclin E1 Recombinant Rabbit Monoclonal Antibody - 1 Fig : Immunohistochemical analysis of paraffin-embedded Mouse-Cerebellum tissue with Rabbit anti-Cyclin E1 antibody (AWA11321) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11321) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
    Cyclin E1 Recombinant Rabbit Monoclonal Antibody - 2 Fig: Fluorescence immunohistochemical analysis of Rat-cerebellum tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-Cyclin E1 antibody (AWA11321) at 1/200 dilution.

    The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11321) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (red). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.
    在线客服
    关闭
    小薇 薇薇 小艾 小奥
    联系销售
    技术支持

    image.png

    月琴艾碧维二维码.jpg