Cyclin E1 Recombinant Rabbit Monoclonal Antibody
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- 20μL
- ¥620
- 1-3个工作日
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- 50μL
- ¥1250
- 1-3个工作日
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- 100μL
- ¥2200
- 1-3个工作日
Product Details | Host Species: Rabbit | Reactivity: Human,Mouse,Rat | Molecular Wt: 47 kDa | |
Clonality: Monoclonal | Isotype: IgG | Concentration: 1mg/ml | ||
Other Names: CCNE; CCNE1; Ccne1; cyclin E1; Cyclin Es; Cyclin Et; CyclinE; G1/S specific cyclin E1; G1/S specific cyclin E | ||||
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. | ||||
Purification: Affinity-chromatography | ||||
Storage: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage. | ||||
Applications | IHC-P 1:200-1000 | |||
Immunogen Information | Gene Name: CCNE1 | Protein Name: G1/S-specific cyclin-E1 | ||
Gene ID: 898 (Human) | SwissPro: P24864 (Human) | |||
Subcellular Location: Nucleus. | ||||
Immunogen: Synthetic peptide within human Cyclin E1. AA range: 361-410. | ||||
Specificity: Cyclin E1 Monoclonal Antibody detects endogenous levels of Cyclin E1 protein. | ||||
Product images | |
Fig : Immunohistochemical analysis of paraffin-embedded Mouse-Cerebellum tissue with Rabbit anti-Cyclin E1 antibody (AWA11321) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11321) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig: Fluorescence immunohistochemical analysis of Rat-cerebellum tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-Cyclin E1 antibody (AWA11321) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11321) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (red). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner. |
-
-
- 20μL
- ¥620
- 1-3个工作日
-
- 50μL
- ¥1250
- 1-3个工作日
-
- 100μL
- ¥2200
- 1-3个工作日
-
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