CD206 Recombinant Rabbit Monoclonal Antibody

货号:
AWA11319
应用:
WB,IF-C,FCM
反应性:
Human,Mouse,Rat
来源:
Rabbit
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

    Host Species:

    Rabbit

    Reactivity:

    Human,Mouse,Rat

    Molecular Wt:

    166 kDa


    Clonality:

    Monoclonal

    Isotype:

    IgG

    Concentration:

    1mg/ml


    Other Names:

    MRC1; CLEC13D; CLEC13DL; MRC1L1; Macrophage mannose receptor 1; MMR; C-type lectin domain family 13 member D; C-type lectin domain family 13 member D-like; Macrophage mannose receptor 1-like protein 1; CD206


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.



    Applications

    WB 1:1000-1:5000
    IF-C 1:50-1:100
    FCM 1:500-1:1000



    Immunogen

    Information

    Gene Name:

    MRC1

    Protein Name:

    Macrophage mannose receptor 1


    Gene ID:

    4360 (Human)    
    17533 (Mouse)

    SwissPro:

    P22897 (Human)    
    Q61830 (Mouse)


    Subcellular Location:

    Endosome membrane. Cell membrane.


    Immunogen:

    Synthetic peptide within human CD206. AA range: 1407-1456.


    Specificity:

    CD206 Monoclonal Antibody detects endogenous levels of CD206 protein.




    Product images
    CD206 Recombinant Rabbit Monoclonal Antibody - 1 Fig : Western blot analysis of CD206 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody ( AWA11319, 1/1000) was used in TBST(0.2%TWEEN20) at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.(6% SDS-PAGE gel.)
    Positive control:
    Lane 1: PC12 cell
    Lane 2: NRK-49F cell
    Lane 3: RBL-2H3 cell
    Lane 4: Mouse spleen
    Lane 5: HSC-T6 cell
    Lane 6: AML12 cell

    Predicted molecular weight: 166 kDa
    Observed molecular weight: 160-180 kDa,59-65 kDa(Isoform)

    Exposure time: 45 seconds
    CD206 Recombinant Rabbit Monoclonal Antibody - 2 Fig : Western blot analysis of CD206 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody ( AWA11319, 1/1000) was used in TBST(0.2%TWEEN20) at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.(6% SDS-PAGE gel.)
    Positive control:
    Lane 1: NIH/3T3 cell
    Lane 2: K562 cell
    Lane 3: A549 cell
    Lane 4: HEK293 cell
    Lane 5: COS7 cell
    Lane 6: Hela cell
    Lane 7: Jurkat cell
    Lane 8: HepG2 cell
    Lane 9: HEPA1-6 cell

    Predicted molecular weight: 166 kDa
    Observed molecular weight: 160-180 kDa, 59-65 kDa(Isoform)

    Exposure time: 7 seconds
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