NFκB-p105/p50 Recombinant Rabbit Monoclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA11307

应用：

WB,IHC-P,IF-T,FCM

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ￥620 1-3个工作日
50μL ￥1250 1-3个工作日
100μL ￥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat

Molecular Wt:

105/50 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

DNA binding factor KBF1; EBP 1; KBF1; NF kappa B; NFKB p105; NFKB p50; NFKB1; NFKB1; p105; NFKB1; p105; p50; nfkb1a; NF-κB; NF-κB 1; p105; p50

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:1000-1:5000

IHC-P 1:50-1:1000

IF-T 1:50-1:200

FCM 1:1000

Immunogen

Information

Gene Name：

NFKB1

Protein Name：

Nuclear factor NF-kappa-B p105 subunit

Gene ID：

4790 (Human)

18033 (Mouse)

81736 (Rat)

SwissPro：

P19838 (Human)

P25799 (Mouse)

Q63369 (Rat)

Subcellular Location：

Cytoplasm, Nucleus.

Immunogen：

Synthetic peptide within human NFkB p105/p50. AA range: 330-370.

Specificity:

NFkB p105/p50 Monoclonal Antibody detects endogenous levels of NFkB p105/p50 protein.

Product images
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue with Rabbit anti-NFκB-p105-p50 (AWA11307) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11307) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig: Fluorescence immunohistochemical analysis of Mouse-Small intestine tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-NFκB-p105/p50 antibody (AWA11307) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0688). The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11307) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (green). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.
	Fig : Western blot analysis of NFκB-p105/p50 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA11307, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: NIH3T3 cell Lane 2: MOLT4 cell Lane 3: MCF-7 cell Lane 4: Hela cell Predicted molecular weight:105/50 kDa Observed molecular weight:105/50 kDa Exposure time: 7 seconds