ATF4 Recombinant Rabbit Monoclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA11304

应用：

WB,IHC-P,IF-C,IF-T,FCM,IP

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ￥620 1-3个工作日
50μL ￥1250 1-3个工作日
100μL ￥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat

Molecular Wt:

39 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

ATF4; CREB2; CREB-2; TXREB; Cyclic AMP-dependent transcription factor ATF-4; cAMP-dependent transcription factor ATF-4; Activating transcription factor 4; Cyclic AMP-responsive element-binding protein 2; CREB-2; cAMP-responsive element-binding prot

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:500-1:2000

IHC-P 1:50-1:1000

IF-C 1:50-1:200

IF-T 1:50-1:200

FCM 1:50-1:100

IP Use at an assay dependent concentration.

Immunogen

Information

Gene Name：

ATF4

Protein Name：

Cyclic AMP-dependent transcription factor ATF-4

Gene ID：

468 (Human)

11911 (Mouse)

79255 (Rat)

SwissPro：

P18848 (Human)

Q06507 (Mouse)

Q9ES19 (Rat)

Subcellular Location：

Nucleus, Nucleus speckle, Cell membrane, Cytoplasm, Centrosome.

Immunogen：

Recombinant protein within Human ATF4. AA range: 1-220.

Specificity:

ATF4 Monoclonal Antibody detects endogenous levels of ATF4 protein.

Product images
	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-ileum tissue with Rabbit anti-ATF4 (AWA11304) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11304) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-brain tissue with Rabbit anti-ATF4 (AWA11304) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11304) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-testis tissue with Rabbit anti-ATF4 (AWA11304) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11304) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Western blot analysis of ATF4 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA11304, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: T24 cell Lane 2: SK-BR-3 cell Predicted molecular weight:39KD Observed molecular weight:42KD Exposure time：3 min