Flt-1 Recombinant Rabbit Monoclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA11303

应用：

WB,IHC-P,IF-C,IF-T,FCM,IP

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat

Molecular Wt:

151 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

FLT; FLT 1; FLT1; FLT-1; Fms like tyrosine kinase 1; FRT; sflt1; Tyrosine protein kinase FRT; VEGF receptor 1; VEGFR 1; VEGFR1; VEGFR-1; VEGFR-1/FLT-1

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:1000-1:5000

IHC-P 1:50-1:200

IF-C 1:50-1:100

IF-T 1:50-1:100

FCM 1:50-1:100

IP Use at an assay dependent concentration.

Immunogen

Information

Gene Name：

FLT1

Protein Name：

Vascular endothelial growth factor receptor 1

Gene ID：

2321 (Human)

14254 (Mouse)

54251 (Rat)

SwissPro：

P17948 (Human)

P35969 (Mouse)

P53767 (Rat)

Subcellular Location：

Cell membrane. Endosome. Secreted. Cytoplasm.

Immunogen：

Synthetic peptide within Human VEGF Receptor 1. AA range: 1-50.

Specificity:

Flt-1 Monoclonal Antibody detects endogenous levels of Flt-1 protein.

Product images
	Fig : Western blot analysis of Flt-1 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA11303, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SH-SY5Y cell Lane 2: Hela cell Lane 3: HT22 cell Lane 4: Rat Heart Lane 5: Rat Brain Lane 6: N-2a cell Lane 7: Hek293 cell Lane 8: U87-MG cell Exposure time: 7 seconds Predicted band size: 151 kDa Observed band size: 151 kDa
	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-spleen tissue with Rabbit anti-Flt-1 (AWA11303) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11303) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-lung tissue with Rabbit anti-Flt-1 (AWA11303) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11303) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.