ULK1 Recombinant Rabbit Monoclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA11295

应用：

WB,IHC-P,IF-C,IF-T

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ￥620 1-3个工作日
50μL ￥1250 1-3个工作日
100μL ￥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat

Molecular Wt:

113 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

ATG1; FLJ38455; FLJ46475; KIAA0722; ULK1; Unc 51 like kinase 1; UNC51; Unc51.1; ATG1A; hATG1

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:1000-1:5000

IHC-P 1:50-1:200

IF-C 1:50-1:200

IF-T 1:50-1:200

Immunogen

Information

Gene Name：

ULK1

Protein Name：

Serine/threonine-protein kinase ULK1

Gene ID：

8408 (Human)

22241 (Mouse)

360827 (Rat)

SwissPro：

O75385 (Human)

O70405 (Mouse)

A6J290 (Rat)

Subcellular Location：

Cytoplasm, cytosol. Preautophagosomal structure.

Immunogen：

Synthetic peptide within human ULK1. AA range: 930-979.

Specificity:

ULK1 Monoclonal Antibody detects endogenous levels of ULK1 protein.

Product images
	Fig: Immunocytochemistry analysis of HEK-293 cells labeling ULK1 with Rabbit anti-ULK1 antibody （AWA11295）at 1/50 dilution(Green). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-ULK1 antibody （AWA11295）at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, AWS0005c) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).
	Fig: Fluorescence immunohistochemical analysis of rat-myocardium tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-ULK1 antibody (AWA11295) at 1/100 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0688). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody ( AWA11295 ) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (green). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.
	Fig: Fluorescence immunohistochemical analysis of rat-spleen tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-ULK1 antibody (AWA11295) at 1/100 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0688). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody ( AWA11295 ) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (green). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.
	Fig : Western blot analysis of ULK1 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA11295, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell Lane 2: Hela cell Lane 3: U87-MG cell Lane 4: MCF-7 cell Lane 5: THP-1 cell Lane 6: 4T1 cell Lane 7: RBL-2H3 cell Predicted molecular weight:113 kDa Observed molecular weight:125 kDa Exposure time：90seconds