Insulin Recombinant Rabbit Monoclonal Antibody

货号:
AWA11213
应用:
IHC-P,IF-C,IF-T,mIHC
反应性:
Human,Mouse,Rat
来源:
Rabbit
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

     

    Host Species:

    Rabbit

    Reactivity:

    Human,Mouse,Rat

    Molecular Wt:

    12 kDa


    Clonality:

    Monoclonal 

    Isotype:

    IgG

    Concentration:

    1 mg/ml


    Other Names:

    IDDM; ILPR; IRDN; IDDM1; IDDM2; PNDM4; MODY10; Insulin; INS


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    -20°C,1 year



    Applications

     

    IHC-P 1:200-1:1000

    IF-C 1:50-1:500

    IF-T 1:50-1:500

    mIHC 1:200-1:8000 

     



    Immunogen

    Information

    Gene Name:

    INS

    Protein Name:

    Insulin


    Gene ID:

    3630 (Human)     

    16333 (Mouse)

    24505 (Rat)

    SwissPro:

    P01308 (Human)     

    P01325 (Mouse)     

    P01322 (Rat)

     


    Subcellular Location:

    Secreted.


    Immunogen:

    Recombinant protein within human Insulin. AA range: 15-110.


    Specificity:

    Insulin Monoclonal Antibody detects endogenous levels of Insulin protein.


    Product images
    Insulin Recombinant Rabbit Monoclonal Antibody - 1
    Insulin Recombinant Rabbit Monoclonal Antibody - 2 Fig: Immunocytochemistry analysis of β-TC-6 cells labeling insulin with Rabbit anti-insulin antibody (AWA11213) at 1/50 dilution(green). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-insulin antibody (AWA11213) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™488, AWS0005) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).
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