ASGR1 Recombinant Rabbit Monoclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA11030

应用：

WB,IHC-P,IF-C,IF-T,FCM,IP

反应性：

Human,Rat

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Rat

Molecular Wt:

33 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

ASGP R 1; ASGP-R 1; ASGPR; ASGPR 1; Asgpr1; ASGR1; asialoglycoprotein receptor 1; CLEC4H1; Hepatic lectin H1; HL 1; HL-1; Hs.12056

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:1000

IHC-P 1:200-1:4000

IF-C 1:50-1:100

IF-T 1:1000

FCM 1:1000

IP 1-2μg/sample

Immunogen

Information

Gene Name：

ASGR1

Protein Name：

Asialoglycoprotein receptor 1

Gene ID：

432 (Human)

24210 (Rat)

SwissPro：

P07306 (Human)

P02706 (Rat)

Subcellular Location：

Membrane. Secreted.

Immunogen：

Recombinant protein within human ASGR1. AA range: 41-291.

Specificity:

ASGR1 Monoclonal Antibody detects endogenous levels of ASGR1 protein.

Product images
	Fig : Western blot analysis of ASGR1 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA11030, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HEPG2 cell Lane 2: HEP3B2.1-7 cell Lane 3: Rat liver Predicted molecular weight:33 kDa Observed molecular weight:33 kDa Exposure time：7 sec
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-liver tissue with Rabbit anti-ASGR1 antibody (AWA11030) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11030) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig: Fluorescence immunohistochemical analysis of rat-liver tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-ASGR1 antibody (AWA11030) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0691). The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11030) at 1/200 dilution for 2 hour at 37℃ or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (Purple). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.