BTF3 Rabbit Monoclonal Antibody

货号:
AWA11027
应用:
WB,IHC,IP
反应性:
Human,Mouse,Rat
来源:
Rabbit
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

     

    Host Species:

    Rabbit

    Reactivity:

    Human,Mouse,Rat

    Molecular Wt:

    22 kDa


    Clonality:

    Monoclonal

    Isotype:

    IgG

    Concentration:

    1 mg/ml


    Other Names:

    NACB; BTF3a; BTF3b; BETA-NAC; BTF3


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    -20°C,1 year



    Applications

     

    WB 1:500-1:1000

    IHC 1:50-1:100

    IP 1:20

     



    Immunogen

    Information

    Gene Name:

    BTF3

    Protein Name:

    Transcription factor BTF3


    Gene ID:

    689 (Human)     

    218490 (Mouse)     

    294680 (Rat)

     

    SwissPro:

    P20290 (Human)     

    Q64152 (Mouse)     

    A6I541 (Rat)

     


    Subcellular Location:

    Cytoplasm. Nucleus.


    Immunogen:

    Recombinant protein of human BTF3.


    Specificity:

    BTF3 Monoclonal Antibody detects endogenous levels of BTF3 protein.


    Product images
    BTF3 Rabbit Monoclonal Antibody - 1 Fig : Western blot analysis of BTF3 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA11027, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti- Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: A431 cell
    Lane 2: HEK293 cell
    Lane 3: HepG2 cell
    Lane 4: MCF-7 cell
    Lane 5: NIH/3T3 cell
    Lane 6: RAW 264.7 cell

    Predicted molecular weight:22 kDa
    Observed molecular weight:22 kDa

    Exposure time: 45 seconds
    BTF3 Rabbit Monoclonal Antibody - 2 Fig : Western blot analysis of BTF3 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA11027, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti- Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: HeLa cell
    Lane 2: Jurkat cell
    Lane 3: THP-1 cell
    Lane 4: Mouse spleen tissue
    Lane 5: RBL-2H3 cell
    Lane 6: PC12 cell
    Lane 7: Rat heart tissue

    Predicted molecular weight:22kDa
    Observed molecular weight:22kDa

    Exposure time: 180 seconds
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