METTL3 Recombinant Rabbit Monoclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA11026

应用：

WB,IHC-P,IF

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat

Molecular Wt:

64 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

IME4; methyltransferase like 3; Methyltransferase-like protein 3; METTL3; MT A70; MT-A70; MTA70; Spo8; M6A; mRNA m(6)A methyltransferase; N6 adenosine methyltransferase 70 kDa subunit

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:1000

IHC-P 1:50-1:1000

IF 1:50-1:100

Immunogen

Information

Gene Name：

METTL3

Protein Name：

N6-adenosine-methyltransferase catalytic subunit

Gene ID：

56339 (Human)

56335 (Mouse)

361035 (Rat)

SwissPro：

Q86U44 (Human)

Q8C3P7 (Mouse)

Q4V8G6 (Rat)

Subcellular Location：

Nucleus. Nucleus speckle. Cytoplasm.

Immunogen：

Recombinant protein within human METTL3. AA range: 1-300.

Specificity:

METTL3 Monoclonal Antibody detects endogenous levels of METTL3 protein.

Product images
	Fig : Western blot analysis of METTL3 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA11026, 1/2000) was used in TBST at room temperature for 2 hours. Goat AntiRabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1:5637 cell Lane 2:LN229 cell Lane 3: SK-BR-3 cell Lane 4: HELA cell Lane 5: SW480 cell Lane 6: AGS cell Lane 7: CAKI-1 cell Lane 8: THP-1 cell Predicted molecular weight:64KD Observed molecular weight:75KD Exposure time：90seconds
	Fig: Fluorescence immunohistochemical analysis of Rat-cerebellum tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-METTL3 antibody (AWA11026) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0691). The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11026) at 1/200 dilution for 2 hour at 37℃ or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (Purple). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.