PDGFB Recombinant Rabbit Monoclonal Antibody
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- 20μL
- ¥620
- 1-3个工作日
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- 50μL
- ¥1250
- 1-3个工作日
-
- 100μL
- ¥2200
- 1-3个工作日
Product Details | Host Species: Rabbit | Reactivity: Human,Mouse,Rat | Molecular Wt: 27 kDa | |
Clonality: Monoclonal | Isotype: IgG | Concentration: 1mg/ml | ||
Other Names: Becaplermin; c sis; FLJ12858; PDGF 2; PDGF-2; PDGF subunit B; PDGF2; PDGFB; Proto oncogene c Sis; SIS; SSV; Oncogene SIS; PDGF B chain; PDGF Beta | ||||
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. | ||||
Purification: Affinity-chromatography | ||||
Storage: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage. | ||||
Applications | IF-T 1:100-1:500 | |||
Immunogen Information | Gene Name： PDGFB | Protein Name： Platelet-derived growth factor subunit B | ||
Gene ID： 5155 (Human) | SwissPro： P01127 (Human) | |||
Subcellular Location： Secreted. | ||||
Immunogen： Synthetic peptide within human PDGFB. AA range: 192-241. | ||||
Specificity: PDGFB Monoclonal Antibody detects endogenous levels of PDGFB protein. | ||||
| Product images | |
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Fig: Fluorescence immunohistochemical analysis of mouse-brain tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-PDGFB antibody (AWA11021) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0691). The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11021) at 1/200 dilution for 2 hour at 37℃ or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (Purple). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner. |
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Fig: Fluorescence immunohistochemical analysis of mouse-heart muscle tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-PDGFB antibody (AWA11021) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0691). The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11021) at 1/200 dilution for 2 hour at 37℃ or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (Purple). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner. |
-
-
- 20μL
- ¥620
- 1-3个工作日
-
- 50μL
- ¥1250
- 1-3个工作日
-
- 100μL
- ¥2200
- 1-3个工作日
-
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