Hsc70 Recombinant Rabbit Monoclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA11019

应用：

WB,IHC-P,IF-C,IF-T,IP

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat

Molecular Wt:

71 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

Heat shock 70 kDa protein 8; heat shock 70kDa protein 8; Heat shock cognate protein 71 kDa; HSC54; HSC70; HSC71; HSP71; NIP71; HSP73, HSP7C; HSPA10; HSPA8; LAP1; LAP-1; HEL-33; HEL-S-72p; Hsc70/HSPA8

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:1000

IHC-P 1:50-1:200

IF-C 1:50

IF-T 1:50

IP Use at an assay dependent concentration.

Immunogen

Information

Gene Name：

HSPA8

Protein Name：

Heat shock cognate 71 kDa protein

Gene ID：

3312 (Human)

15481 (Mouse)

24468 (Rat)

SwissPro：

P11142 (Human)

P63017 (Mouse)

P63018 (Rat)

Subcellular Location：

Cytoplasm. Melanosome. Nucleus, nucleolus. Cell membrane. Lysosome membrane.

Immunogen：

Synthetic peptide within Human Hsc70. AA range: 597-646.

Specificity:

Hsc70 Monoclonal Antibody detects endogenous levels of Hsc70 protein.

Product images
	Fig : Western blot analysis of Hsc70 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA11019, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: T24 cell Lane 2: 143B cell Lane 3: U87-MG cell Lane 4: Mouse brain Lane 5: Rat brain Lane 6: MDA-MB-231 cell Lane 7: 4T1 cell Lane 8: SHZ-88 cell Lane 9: C-33A cell Predicted molecular weight:71 kDa Observed molecular weight:71 kDa Exposure time：90 sec
	Fig : Western blot analysis of Hsc70 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA11019, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HCT116 cell Lane 2: CT26 cell Lane 3: CAKI-1 cell Lane 4: NRK-49F cell Lane 5: A549 cell Lane 6: LLC cell Lane 7: Rat lung Predicted molecular weight:71 kDa Observed molecular weight:71 kDa Exposure time：90 sec