Cyclin A2 Recombinant Rabbit Monoclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA11009

应用：

WB,IHC-P,IF-C,IF-T

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat

Molecular Wt:

49 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

CCN1; CCNA; CCNA2; Cyclin A; Cyclin-A; Cyclin A2; Cyclin-A2; cyclinA2

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:500-1:2000

IHC-P 1:50-1:200

IF-C 1:100-1:500

IF-T 1:100-1:500

Immunogen

Information

Gene Name：

CCNA2

Protein Name：

Cyclin-A2

Gene ID：

890 (Human)

12428 (Mouse)

114494 (Rat)

SwissPro：

P20248 (Human)

P51943 (Mouse)

Q91ZX8 (Rat)

Subcellular Location：

Nucleus. Cytoplasm.

Immunogen：

Recombinant protein within mouse Cyclin A2. AA range: 1-240.

Specificity:

Cyclin A2 Monoclonal Antibody detects endogenous levels of Cyclin A2 protein.

Product images
	Fig: Immunocytochemistry analysis of U2OS cells labeling Cyclin A2 with Rabbit anti-Cyclin A2 antibody (AWA11009) at 1/50 dilution(green ). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-Cyclin A2 antibody (AWA11009) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, AWS0005) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).
	Fig : Western blot analysis of Cyclin A2 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA11009, 1/4000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: K562 cell Lane 2: HEPG2 cell Lane 3: Jurkat cell Lane 4: MCF-7 cell Lane 5: T24 cell Lane 6: SHZ-88 cell Lane 7: HEPA1-6 cell Lane 8: Mouse testicles Lane 9: 4T1 cell Predicted molecular weight:48KD Observed molecular weight:48KD Exposure time：15 sec
	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-spleen tissue with Rabbit anti-Cyclin A2 antibody (AWA11009) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11009) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig: Fluorescence immunohistochemical analysis of mouse-Lewis cell derived xenograft tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-Cyclin A2 antibody (AWA11009) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0691). The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11009) at 1/200 dilution for 2 hour at 37℃ or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (Purple). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.