GRIA1 Recombinant Rabbit Monoclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA11005

应用：

WB,IHC-P,IHC-F,IP

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat

Molecular Wt:

102 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

GluA1; GLUH1; GluR 1; GluR-1; GluR A; GluR-A; GluR K1; GluR-K1; GluRK1; GLUR1; GLUR 1; GLURA; GLUR A; Glutamate receptor 1; Gria1; GRIA1; GRIA1_HUMAN; HBGR1; AMPA 1; AMPA selective glutamate receptor 1; AMPA-selective glutamate receptor 1; Glutamate receptor ionotropic AMPA 1; MGC133252

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:1000

IHC-P 1:50-1:200

IHC-F 1:200

IP Use at an assay dependent concentration.

Immunogen

Information

Gene Name：

GRIA1

Protein Name：

Glutamate receptor 1

Gene ID：

2890 (Human)

14799 (Mouse)

50592 (Rat)

SwissPro：

P42261 (Human)

P23818 (Mouse)

P19490 (Rat)

Subcellular Location：

Cell membrane. Endoplasmic reticulum membrane. Postsynaptic cell membrane. Postsynaptic density membrane. Cell projection, dendrite. Cell projection, dendritic spine. Early endosome membrane. Recycling endosome membrane. Presynapse. Synapse.

Immunogen：

Synthetic peptide within Human Glutamate receptor 1. AA range: 857-906.

Specificity:

GRIA1 Monoclonal Antibody detects endogenous levels of GRIA1 protein.

Product images
	Fig : Western blot analysis of GRIA1 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody ( AWA11005, 1/1000) was used in TBST(0.4%TWEEN20) at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.(10% SDS-PAGE gel.) Positive control: Lane 1: Mouse brain tissue Predicted molecular weight: 101.5 kDa Observed molecular weight: 101.5 kDa Exposure time: 45 seconds
	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-hippocampus tissue with Rabbit anti-GRTA antibody (AWA11005) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11005) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.