SERCA2 Recombinant Rabbit Monoclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA11002

应用：

WB,IHC-P,IF-C,IF-T,FCM

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat

Molecular Wt:

115 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

ATP2A2; Atp2a2; ATP2B; Calcium pump 2; DAR; DD; SERCA2; SERCA2; SR Ca(2+) ATPase 2; Cardiac Ca2+ ATPase; AT2A2_HUMAN

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:1000

IHC-P 1:50-1:200

IF-C 1:50-1:200

IF-T 1:50-1:200

FCM 1:50-1:100

Immunogen

Information

Gene Name：

ATP2A2

Protein Name：

Sarcoplasmic/endoplasmic reticulum calcium ATPase 2

Gene ID：

488 (Human)

11938 (Mouse)

29693 (Rat)

SwissPro：

P16615 (Human)

O55143 (Mouse)

P11507 (Rat)

Subcellular Location：

Endoplasmic reticulum membrane. Sarcoplasmic reticulum membrane.

Immunogen：

Synthetic peptide within Human SERCA2. AA range: 999-1042.

Specificity:

SERCA2 Monoclonal Antibody detects endogenous levels of SERCA2 protein.

Product images
	Fig: Fluorescence immunohistochemical analysis of mouse-brain tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-SERCA2 antibody (AWA11002) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11002) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (red). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.
	Fig: Fluorescence immunohistochemical analysis of mouse-testis tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-SERCA2 antibody (AWA11002) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11002) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (red). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.
	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-cerebellum tissue with Rabbit anti-SERCA2 antibody (AWA11002) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11002) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.