CD68 Recombinant Rabbit Monoclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA11001

应用：

WB,IHC-P,IHC-F,IF-C,IF-T

反应性：

Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Mouse,Rat

Molecular Wt:

37 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

Cd68; CD 68; CD68; CD68_HUMAN; CD68 antigen; CD68 molecule; gp110; Scard1; LAMP4; Macrosialin

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:1000

IHC-P 1:2000

IHC-F 1:200

IF-C 1:100

IF-T 1:200

Immunogen

Information

Gene Name：

CD68

Protein Name：

Macrosialin

Gene ID：

968 (Human)

12514 (Mouse)

287435 (Rat)

SwissPro：

P34810 (Human)

P31996 (Mouse)

Q4FZY1 (Rat)

Subcellular Location：

Cell membrane. Endosome membrane. Lysosome membrane.

Immunogen：

Recombinant protein within.

Specificity:

CD68 Monoclonal Antibody detects endogenous levels of CD68 protein.

Product images
	Fig : Western blot analysis of CD68 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA11001, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: RAW264.7 cell Lane 2: J774A.1 cell Lane 3: Rat spleen tissue Predicted molecular weight:35KD Observed molecular weight:90-100KD Exposure time:90 sec
	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-spleen tissue with Rabbit anti-CD68 antibody (AWA11001) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11001) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue with Rabbit anti-CD68 antibody (AWA11001) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11001) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.