NQO1 Recombinant Rabbit Monoclonal Antibody

货号:
AWA10998
应用:
WB,IF,FCM,IP
反应性:
Human,Mouse,Rat
来源:
Rabbit
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

     

    Host Species:

    Rabbit

    Reactivity:

    Human,Mouse,Rat

    Molecular Wt:

    31 kDa


    Clonality:

    Monoclonal 

    Isotype:

    IgG

    Concentration:

    1 mg/ml


    Other Names:

    Azoreductase; DHQU; DIA4; DIA 4; DT diaphorase; DT-diaphorase; DTD; Menadione reductase; NQO1; NQO 1; NQO1_HUMAN; Phylloquinone reductase; QR1; QR 1; Quinone reductase 1

     


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    -20°C,1 year



    Applications

     

    WB 1:1000-1:5000

    IF 1:50-1:200

    FCM 1:50-1:100

    IP Use at an assay dependent concentration.

     



    Immunogen

    Information

    Gene Name:

    NQO1

    Protein Name:

    NAD(P)H dehydrogenase [quinone] 1


    Gene ID:

    1728 (Human)     

    18104 (Mouse)     

    24314 (Rat)

     

    SwissPro:

    P15559 (Human)     

    Q64669 (Mouse)     

    P05982 (Rat)

     


    Subcellular Location:

    Cytoplasm, cytosol.


    Immunogen:

    Synthetic peptide within Human NQO1. AA range: 1-49.


    Specificity:

    NQO1 Monoclonal Antibody detects endogenous levels of NQO1 protein.




    Product images
    NQO1 Recombinant Rabbit Monoclonal Antibody - 1 Fig : Western blot analysis of NQO1 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA10998, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti- Rabbit IgG - HRP Secondary Antibody (/AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: A431cell
    Lane 2: Hela cell
    Lane 3: HepG2 cell
    Lane 4: A549 cell
    Lane 5: PC-3 cell
    Lane 6: SHZ-88 cell
    Lane 7: PC12 cell
    Lane 8: HSC-T6 cell

    Predicted molecular weight:31KD
    Observed molecular weight:31KD

    Exposure time: 7 seconds
    NQO1 Recombinant Rabbit Monoclonal Antibody - 2 Fig : Western blot analysis of NQO1 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA10998, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti- Rabbit IgG - HRP Secondary Antibody (/AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: NIH/3T3 cell
    Lane 2: C2C12 cell
    Lane 3: Mouse kidney tissue
    Lane 4: Mouse heart tissue

    Predicted molecular weight:31KD
    Observed molecular weight:31KD

    Exposure time: 45 seconds
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