BMAL1 Recombinant Rabbit Monoclonal Antibody

货号:
AWA10992
应用:
WB,IF-T
反应性:
Human,Mouse,Rat
来源:
Rabbit
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

    Host Species:

    Rabbit

    Reactivity:

    Human,Mouse,Rat

    Molecular Wt:

    69 kDa


    Clonality:

    Monoclonal

    Isotype:

    IgG

    Concentration:

    1mg/ml


    Other Names:

    ARNT3; ARNTL; bHLH PAS protein JAP3; bHLH-PAS protein JAP3; bHLHe5; BMAL1; BMAL 1; BMAL1c; Brain and muscle ARNT like 1; Brain and muscle ARNT-like 1; JAP 3 ; JAP3; Member of PAS protein 3; Member of PAS superfamily 3; MOP 3; MOP3; PASD 3; PASD3; TIC


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.



    Applications

    WB 1:500-1:2000
    IF-T 1:100-1:500



    Immunogen

    Information

    Gene Name:

    BMAL1

    Protein Name:

    Basic helix-loop-helix ARNT-like protein 1


    Gene ID:

    406 (Human)
    11865 (Mouse)
    29657 (Rat)

    SwissPro:

    O00327 (Human)
    Q9WTL8 (Mouse)
    Q9EPW1 (Rat)


    Subcellular Location:

    Nucleus. Cytoplasm. Nucleus, PML body.


    Immunogen:

    Synthetic peptide within human BMAL1. AA range: 550-599.


    Specificity:

    BMAL1 Monoclonal Antibody detects endogenous levels of BMAL1 protein.




    Product images
    BMAL1 Recombinant Rabbit Monoclonal Antibody - 1 Fig : Western blot analysis of BMAL1 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA10992, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti- Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: SH-SY5Y cell
    Lane 2: HeLa cell
    Lane 3: K562 cell
    Lane 4: PC-3 cell
    Lane 5: HT1080 cell
    Lane 6: THP-1 cell
    Lane 7: MCF-7 cell


    Predicted molecular weight:69KD
    Observed molecular weight:69-75KD

    Exposure time: 15 seconds
    BMAL1 Recombinant Rabbit Monoclonal Antibody - 2 Fig : Western blot analysis of BMAL1 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA10992, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti- Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: HT22 cell
    Lane 2: N-2A cell
    Lane 3: NIH/3T3 cell
    Lane 4: Mouse testis tissue
    Lane 5: RSC96 cell
    Lane 6: Rat brain tissue
    Lane 7: Rat liver tissue


    Predicted molecular weight:69KD
    Observed molecular weight:69-75KD

    Exposure time: 15 seconds
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