PARK7/DJ1 Recombinant Rabbit Monoclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA10988

应用：

WB,IHC-P,IF-C,IF-T,FCM,IP

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat

Molecular Wt:

20 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

CAP1; DJ 1; DJ1; DJ-1; Oncogene DJ1; PARK7; PARK7; PARK7_HUMAN; DJ-1; Parkinson disease protein 7; Parkinson protein 7; Protein DJ 1; DJ1 protein; FLJ27376; FLJ34360; FLJ92274; HEL S 67p

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:1000-1:2000

IHC-P 1:100-1:500

IF-C 1:100-1:500

IF-T 1:100-1:500

FCM 1:50-1:100

IP Use at an assay dependent concentration.

Immunogen

Information

Gene Name：

PARK7

Protein Name：

Parkinson disease protein 7

Gene ID：

11315 (Human)

57320 (Mouse)

117287 (Rat)

SwissPro：

Q99497 (Human)

Q99LX0 (Mouse)

O88767 (Rat)

Subcellular Location：

Cell membrane. Cytoplasm. Nucleus. Membrane raft. Mitochondrion. Endoplasmic reticulum.

Immunogen：

Synthetic peptide within Human PARK7. AA range: 11-60.

Specificity:

PARK7/DJ1 Monoclonal Antibody detects endogenous levels of PARK7/DJ1 protein.

Product images
	Fig : Western blot analysis of PARK7/DJ1 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA10988, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti- Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: A431 cell Lane 2: HEK293 cell Lane 3: K562 cell Lane 4: HepG2 cell Lane 5: Mouse brain tissue Lane 6: Mouse heart tissue Predicted molecular weight:20KD Observed molecular weight:20-25KD Exposure time: 15 seconds
	Fig: Fluorescence immunohistochemical analysis of Mouse-brain tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-PARK7 antibody (AWA10988) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0691). The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10988) at 1/200 dilution for 2 hour at 37℃ or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (Purple). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.