TMP21 Recombinant Rabbit Monoclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA10986

应用：

WB,IHC-P,IF-T

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat

Molecular Wt:

25 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

1110014C03Rik; P24(DELTA); p24delta; p24delta1; S31I125; S31III125; TMED 10; TMED10; TMEDA_HUMAN; Tmp 21 I; Tmp-21-I; TMP21; Tmp 21; Transmembrane protein Tmp21

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:1000-1:5000

IHC-P 1:200-1:1000

IF-T 1:200

Immunogen

Information

Gene Name：

TMED10

Protein Name：

Transmembrane emp24 domain-containing protein 10

Gene ID：

10972 (Human)

68581 (Mouse)

84599 (Rat)

SwissPro：

P49755 (Human)

Q9D1D4 (Mouse)

Q63584 (Rat)

Subcellular Location：

Endoplasmic reticulum membrane. Endoplasmic reticulum-Golgi intermediate compartment membrane. Golgi apparatus membrane. Golgi apparatus, cis-Golgi network membrane. Golgi apparatus, trans-Golgi network membrane. Cytoplasmic vesicle, secretory vesicle membrane. Cell membrane. Melanosome.

Immunogen：

Recombinant protein within human TMP21. AA range: 1-200.

Specificity:

TMP21 Monoclonal Antibody detects endogenous levels of TMP21 protein.

Product images
	Fig : Western blot analysis of TMP21 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA10986, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell Lane 2: Raji cell Lane 3: HEK293 cell Lane 4: HepG2 cell Lane 5: THP-1 cell Lane 6: RAW264.7 cell Lane 7: N-2A cell Lane 8: Rat liver tissue Predicted molecular weight:25KD Observed molecular weight:21KD Exposure time:45 sec
	Fig: Fluorescence immunohistochemical analysis of Mouse-brain tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-TMP21 antibody (AWA10986) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0691). The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10986) at 1/200 dilution for 2 hour at 37℃ or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (Purple). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.