GM130 Recombinant Rabbit Monoclonal Antibody
-
-
- 20μL
- ¥620
- 1-3个工作日
-
- 50μL
- ¥1250
- 1-3个工作日
-
- 100μL
- ¥2200
- 1-3个工作日
Product Details | Host Species: Rabbit | Reactivity: Human,Mouse,Rat | Molecular Wt: 113 kDa | |
Clonality: Monoclonal | Isotype: IgG | Concentration: 1mg/ml | ||
Other Names: Golgin subfamily A member 2; 30 kDa cis Golgi matrix protein; 130 kDa cis-Golgi matrix protein; GM130; GM130 autoantigen; Golgin-95; Golgin 95; Cell and organelle markers; GOLGA 2; GOLGA2; Golga2; Golgi Marker; Golgi autoantigen | ||||
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. | ||||
Purification: Affinity-chromatography | ||||
Storage: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage. | ||||
Applications | WB 1:500-1:2000 | |||
Immunogen Information | Gene Name： GOLGA2 | Protein Name： Golgin subfamily A member 2 | ||
Gene ID： 2801 (Human) | SwissPro： Q08379 (Human) | |||
Subcellular Location： Golgi apparatus, cis-Golgi network membrane. Endoplasmic reticulum-Golgi intermediate compartment membrane. Cytoplasm, cytoskeleton, spindle pole. | ||||
Immunogen： Recombinant protein within human GM130. AA range: 1-100. | ||||
Specificity: GM130 Monoclonal Antibody detects endogenous levels of GM130 protein. | ||||
| Product images | |
|
|
Fig : Western blot analysis of GM130 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA10983, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: MCF-7 cell Lane 2: K562 cell Lane 3: Mouse brain Predicted molecular weight: 113 kDa Observed molecular weight: 130 kDa Exposure time:45 sec |
|
|
Fig : Western blot analysis of GM130 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA10983, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell Lane 2: HepG2 cell Lane 3: HEK293 cell Lane 4: A549 cell Predicted molecular weight: 113 kDa Observed molecular weight: 130 kDa Exposure time:180 sec |
|
|
Fig : Immunohistochemical analysis of paraffin-embedded Mouse-brain tissue with Rabbit anti-GM130 antibody (AWA10983) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10983) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
|
Fig : Immunohistochemical analysis of paraffin-embedded Mouse-jejunum tissue with Rabbit anti-GM130 antibody (AWA10983) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10983) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
-
-
- 20μL
- ¥620
- 1-3个工作日
-
- 50μL
- ¥1250
- 1-3个工作日
-
- 100μL
- ¥2200
- 1-3个工作日
-
相关产品
-
Cdk6 Recombinant Rabbit Monoclonal Antibody
GAPDH Rabbit Polyclonal Antibody
GFAP Recombinant Mouse Monoclonal Antibody
Ki67 Rabbit Monoclonal Antibody
Stathmin 1 Recombinant Rabbit Monoclonal Antibody
HMGB1 Recombinant Rabbit Monoclonal Antibody
SQSTM1/p62 Mouse Monoclonal Antibody
p53 Recombinant Rabbit Monoclonal Antibody
