NDRG1 Recombinant Rabbit Monoclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA10981

应用：

WB,IHC-P,IF-C,IF-T,IP

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat

Molecular Wt:

43 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

CAP43; cap43; CMT4D; cmt4d; DRG 1; DRG-1; drg1; DRG1; gc4; GC4; hmsnl; HMSNL; N myc downstream regulated 1; NDR1; NDRG1; Protein NDRG1; NDRG1_HUMAN; nmsl; PROXY1; Proxy1; RIT42; Rit42; RTP; TARG1; targ1; TDD5; tdds

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:500-1:2000

IHC-P 1:50-1:200

IF-C 1:50-1:200

IF-T 1:50-1:200

IP 1:50

Immunogen

Information

Gene Name：

NDRG1

Protein Name：

Protein NDRG1

Gene ID：

10397 (Human)

17988 (Mouse)

299923 (Rat)

SwissPro：

Q92597 (Human)

Q62433 (Mouse)

Q6JE36 (Rat)

Subcellular Location：

Cytoplasm, cytosol. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Nucleus. Cell membrane.

Immunogen：

Synthetic peptide within Human NDRG1. AA range: 345-394.

Specificity:

NDRG1 Monoclonal Antibody detects endogenous levels of NDRG1 protein.

Product images
	Fig : Western blot analysis of NDRG1 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA10981, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: LOVO cell Lane 2: Mouse brain tissue Lane 3: Rat brain tissue Predicted molecular weight:43KD Observed molecular weight:43KD Exposure time:180 sec
	Fig: Fluorescence immunohistochemical analysis of Mouse-kidney tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-NDRG1 antibody (AWA10981) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0691). The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10981) at 1/200 dilution for 2 hour at 37℃ or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (Purple). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.
	Fig: Fluorescence immunohistochemical analysis of Rat-kidney tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-NDRG1 antibody (AWA10981) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0691). The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10981) at 1/200 dilution for 2 hour at 37℃ or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (Purple). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.