Histone H3(Acetyl K23) Recombinant Rabbit Monoclonal Antibody

货号:
AWA10972
应用:
WB,IF
反应性:
Human,Mouse
来源:
Rabbit
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

     

    Host Species:

    Rabbit

    Reactivity:

    Human,Mouse

    Molecular Wt:

    15 kDa


    Clonality:

    Monoclonal 

    Isotype:

    IgG

    Concentration:

    1 mg/ml


    Other Names:

    H3 histone family member E pseudogene; H3 histone family, member A; H3/A; H31_HUMAN; H3F3; H3FA; Hist1h3a; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; HIST3H3; histone 1, H3a; Histone cluster 1, H3a; Histone H3 3 pseudogene; Histone H3.1

     


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    -20°C,1 year



    Applications

     

    WB 1:1000

    IF 1:100



    Immunogen

    Information

    Gene Name:

    H3C1; H3C2; H3C3; H3C4; H3C6;

    H3C7; H3C8; H3C10; H3C11; H3C12 

     

    Protein Name:

    Histone H3.1


    Gene ID:

    8350/8351/8352/8353/8354 (Human)     

    319152/319153/360198/97908 (Mouse)     

     

    SwissPro:

    P68431 (Human)     

    P68433 (Mouse)     

     


    Subcellular Location:

    Nucleus, Chromosome.


    Immunogen:

    Synthetic peptide corresponding to residues surrounding acetyl-Lys23 of human histone H3 protein.

     


    Specificity:

    Histone H3 (Acetyl K23) Monoclonal Antibody detects endogenous levels of Histone H3 (Acetyl K23) protein.



    Product images
    Histone H3(Acetyl K23) Recombinant Rabbit Monoclonal Antibody - 1 Fig : Western blot analysis of Histone H3(Acetyl K23) on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA10972, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5000 dilution was used for 1 hour at room temperature.
    Positive control:
    Positive control:
    Lane 1: 4T1 cell
    Lane 2: RAW264.7 cell

    Predicted molecular weight:15kDa
    Observed molecular weight:15kDa

    Exposure time:15s
    Histone H3(Acetyl K23) Recombinant Rabbit Monoclonal Antibody - 2 Fig : Western blot analysis of Histone H3(Acetyl K23) on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA10972, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5000 dilution was used for 1 hour at room temperature.
    Positive control:
    Positive control:
    Lane 1: K562 cell
    Lane 2: SHZ88 cell
    Lane 3: J774A.1 cell
    Lane 4: NIH3T3 cell
    Lane 5: HEK293 cell
    Lane 6: HepG2 cell
    Lane 7: 3D4-21 cell
    Lane 8: Jurkat cell
    Lane 9: Hela cell

    Predicted molecular weight:15kDa
    Observed molecular weight:15kDa

    Exposure time:90s
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