Histone H3(Acetyl K14) Recombinant Rabbit Monoclonal Antibody

货号：

AWA10971

应用：

WB,IHC-P,IF-C,IF-T,SNAP-ChIP,IP

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat

Molecular Wt:

15 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

H3K14ac

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:500-1:2000

IHC-P 1:500

IF-C 1:100

IF-T 1:200

SNAP-ChIP 1:100

IP Use at an assay dependent concentration.

Immunogen

Information

Gene Name：

H3C1; H3C2; H3C3; H3C4; H3C6;

H3C7; H3C8; H3C10; H3C11; H3C12

Protein Name：

Histone H3.1

Gene ID：

8350/8351/8352/8353/8354 (Human)

319152/319153/360198/97908 (Mouse)

291159 (Rat)

SwissPro：

P68431 (Human)

P68433 (Mouse)

Q6LED0 (Rat)

Subcellular Location：

Nucleus, Chromosome.

Immunogen：

Synthetic peptide within human Histone H3. AA range: 1-50 (acetyl K14).

Specificity:

Histone H3 (Acetyl K14) Monoclonal Antibody detects endogenous levels of Histone H3 (Acetyl K14) protein.

Product images
	Fig : Western blot analysis of Histone H3(Acetyl K14) on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA10971, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: J774A.1 cell lysate Lane 2: MCF-7 cell lysate Predicted molecular weight:15KD Observed molecular weight:15KD Exposure time:45s
	Fig : Western blot analysis of Histone H3(Acetyl K14) on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA10971, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5000 dilution was used for 1 hour at room temperature. Positive control: Positive control: Lane 1: HepG2 cell lysate Lane 2: HEK293 cell lysate Lane 3: NIH-3T3 cell lysate Lane 4: Jurkat cell lysate Lane 5: RAW264.7 cell lysate Lane 6: Hela cell lysate Lane 7: 3D4-21 cell lysate Lane 8: 4T1 cell lysate Lane 9: SHZ-88 cell lysate Lane 10: K562 cell lysate Predicted molecular weight:15KD Observed molecular weight:15KD Exposure time:45sceonds
	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue with Rabbit anti-Histone H3(Acetyl K14) antibody (AWA10971) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10971) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-Brain tissue with Rabbit anti-Histone H3(Acetyl K14) antibody (AWA10971) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10791) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.