Tyrosinase Recombinant Rabbit Monoclonal Antibody

货号:
AWA10964
应用:
WB,IHC-P,IF-C,IF-T
反应性:
Human,Mouse
来源:
Rabbit
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

    Host Species:

    Rabbit

    Reactivity:

    Human,Mouse

    Molecular Wt:

    60 kDa


    Clonality:

    Monoclonal

    Isotype:

    IgG

    Concentration:

    1mg/ml


    Other Names:

    TYR; LB24 AB; LB24-AB; LB24AB; Monophenol monooxygenase; SK29 AB; SK29-AB; SK29AB; Tumor rejection antigen AB; OCA1A; OCAIA; OCA1A; SHEP3; Tyrosinase


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.



    Applications

    WB 1:500-1:2000
    IHC-P 1:50-1:200
    IF-C 1:50-1:200
    IF-T 1:100-1:500



    Immunogen

    Information

    Gene Name:

    TYR

    Protein Name:

    Tyrosinase


    Gene ID:

    7299 (Human)  
    22173 (Mouse)

    SwissPro:

    P14679 (Human)  
    P11344 (Mouse)


    Subcellular Location:

    Melanosome membrane. Melanosome.


    Immunogen:

    Recombinant protein within human Tyrosinase. AA range: 1-340.


    Specificity:

    Tyrosinase Monoclonal Antibody detects endogenous levels of Tyrosinase protein.




    Product images
    Tyrosinase Recombinant Rabbit Monoclonal Antibody - 1 Fig : Western blot analysis of Tyrosinase on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA10964, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: SK-MEL-28 cell

    Predicted molecular weight:60KD
    Observed molecular weight:~80KD

    Exposure time:45 sec
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