CD27 Recombinant Rabbit Monoclonal Antibody
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- 20μL
- ¥620
- 1-3个工作日
-
- 50μL
- ¥1250
- 1-3个工作日
-
- 100μL
- ¥2200
- 1-3个工作日
Product Details | Host Species: Rabbit | Reactivity: Human,Mouse,Rat | Molecular Wt: 29 kDa | |
Clonality: Monoclonal | Isotype: IgG | Concentration: 1mg/ml | ||
Other Names: CD27 antigen; CD27 molecule; CD27L receptor; S152; T cell activation antigen CD27; T14; TNFRSF7; TNFRSF 7; TNFSF7; Tp 55; Tp55; LPFS2; MGC20393; CD 27; CD27 | ||||
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. | ||||
Purification: Affinity-chromatography | ||||
Storage: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage. | ||||
Applications | WB 1:500-1:2000 | |||
Immunogen Information | Gene Name: CD27 | Protein Name: CD27 antigen | ||
Gene ID: 939 (Human) | SwissPro: P26842 (Human) | |||
Subcellular Location: Cell membrane. | ||||
Immunogen: Synthetic peptide corresponding to human Intracellular domain of CD27. AA range: 51-100. | ||||
Specificity: CD27 Monoclonal Antibody detects endogenous levels of CD27 protein. | ||||
Product images | |
Fig : Immunohistochemical analysis of paraffin-embedded Mouse-spleen tissue with Rabbit anti-CD27 antibody (AWA10960) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10960) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig : Western blot analysis of CD27 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA10960, 1/1000) was used in PBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Rat thymus Lane 2: PC-3 cell Lane 3: Raji cell Predicted band size: 29 kDa Observed band size: 50-55 kDa Exposure time:45s |
-
-
- 20μL
- ¥620
- 1-3个工作日
-
- 50μL
- ¥1250
- 1-3个工作日
-
- 100μL
- ¥2200
- 1-3个工作日
-
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