CD27 Recombinant Rabbit Monoclonal Antibody

货号:
AWA10960
应用:
WB,IHC-P,FCM
反应性:
Human,Mouse,Rat
来源:
Rabbit
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

    Host Species:

    Rabbit

    Reactivity:

    Human,Mouse,Rat

    Molecular Wt:

    29 kDa


    Clonality:

    Monoclonal

    Isotype:

    IgG

    Concentration:

    1mg/ml


    Other Names:

    CD27 antigen; CD27 molecule; CD27L receptor; S152; T cell activation antigen CD27; T14; TNFRSF7; TNFRSF 7; TNFSF7; Tp 55; Tp55; LPFS2; MGC20393; CD 27; CD27


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.



    Applications

    WB 1:500-1:2000
    IHC-P 1:100-1:500
    FCM 1:50-1:100



    Immunogen

    Information

    Gene Name:

    CD27

    Protein Name:

    CD27 antigen


    Gene ID:

    939 (Human)    
    21940 (Mouse)    
    500318 (Rat)

    SwissPro:

    P26842 (Human)    
    P41272 (Mouse)    
    Q501W2 (Rat)


    Subcellular Location:

    Cell membrane.


    Immunogen:

    Synthetic peptide corresponding to human Intracellular domain of CD27. AA range: 51-100.


    Specificity:

    CD27 Monoclonal Antibody detects endogenous levels of CD27 protein.




    Product images
    CD27 Recombinant Rabbit Monoclonal Antibody - 1 Fig : Immunohistochemical analysis of paraffin-embedded Mouse-spleen tissue with Rabbit anti-CD27 antibody (AWA10960) at 1/100 dilution.

    The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10960) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
    CD27 Recombinant Rabbit Monoclonal Antibody - 2 Fig : Western blot analysis of CD27 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA10960, 1/1000) was used in PBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: Rat thymus
    Lane 2: PC-3 cell
    Lane 3: Raji cell

    Predicted band size: 29 kDa
    Observed band size: 50-55 kDa

    Exposure time:45s
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