MALT1 Recombinant Rabbit Monoclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA10815

应用：

WB,IHC-P

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat

Molecular Wt:

92 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

Mucosa-associated lymphoid tissue lymphoma translocation protein 1; MLT 1; MLT1; MLT; MALT lymphoma-associated translocation; MALT 1; MALT1; Malt1; IMD12; PCASP1; Caspase like protein; DKFZp434L132; MALT associated translocation; MALT1 paracaspase; Paracaspase-1

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:1000-1:5000

IHC-P 1:200

Immunogen

Information

Gene Name：

MALT1

Protein Name：

Mucosa-associated lymphoid tissue lymphoma translocation protein 1

Gene ID：

10892 (Human)

240354 (Mouse)

SwissPro：

Q9UDY8 (Human)

Q2TBA3 (Mouse)

Subcellular Location：

Cytoplasm, perinuclear region. Nucleus.

Immunogen：

Recombinant protein within human MALT1. AA range: 1-400.

Specificity:

MALT1 Monoclonal Antibody detects endogenous levels of MALT1 protein.

Product images
	Fig : Western blot analysis of MALT1 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA10815, 1/2000) was used in TBST at room temperature for 2 hours. Goat Anti-Ribbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell Lane 2: Hela cell Lane 3: HCT116 cell Predicted molecular weight:92KD Observed molecular weight:92KD; Exposure time: 45seconds
	Fig : Western blot analysis of MALT1 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA10815, 1/2000) was used in TBST at room temperature for 2 hours. Goat Anti-Ribbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: K562 cell Lane 2: Mouse liver Lane 3: Rat liver Lane 4: J774A.7 cell Lane 5: Raw264.7 cell Lane 6: Jurkat cell Lane 7: sw620 cell Lane 8: MC38 cell Lane 9:Rat testis Lane 10:Mouse testis Predicted molecular weight:92KD Observed molecular weight:92KD; Exposure time: 90seconds
	Fig: Fluorescence immunohistochemical analysis of Mouse-spleen tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-MALT1 antibody (AWA10815) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0688). The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10815) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (green). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.
	Fig: Fluorescence immunohistochemical analysis of mouse-testis tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-MALT1 antibody (AWA10815) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10815) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (red). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.
	Fig: Fluorescence immunohistochemical analysis of Mouse-duodenum tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-MALT1 antibody (AWA10815) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0688). The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10815) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (green). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.
	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-spleen tissue with Rabbit anti-MALT1 antibody (AWA10815) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10815) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.