TPH2 Recombinant Rabbit Monoclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA10708

应用：

WB,IHC-P,IHC-F,IP

反应性：

Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Mouse,Rat

Molecular Wt:

56 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

NTPH; ADHD7; TPH2; TPH 2; MGC138871; FLJ37295; MGC138872; Neuronal tryptophan hydroxylase; Tryptophan 5-hydroxylase 2; Tryptophan 5-monooxygenase 2

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:1000-1:2000

IHC-P 1:200-1:500

IHC-F 1:200

IP 1-2μg/sample

Immunogen

Information

Gene Name：

TPH2

Protein Name：

Tryptophan 5-hydroxylase 2

Gene ID：

121278 (Human)

216343 (Mouse)

317675 (Rat)

SwissPro：

Q8IWU9 (Human)

Q8CGV2 (Mouse)

Q8CGU9 (Rat)

Subcellular Location：

Cytosol. neuron projection.

Immunogen：

Recombinant protein.

Specificity:

TPH2 Monoclonal Antibody detects endogenous levels of TPH2 protein.

Product images
	Fig : Western blot analysis of TPH2 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA10708, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Rat brain Lane 2: Mouse brain Lane 3: AML12 cell Predicted molecular weight: 56 kDa Observed molecular weight: 56 kDa Exposure time：90s
	Fig: Immunocytochemistry analysis of NIH/3T3 cells labeling TPH2 with Rabbit anti-TPH2 antibody (AWA10708) at 1/50 dilution(green). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-TPH2 antibody (AWA10708) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, AWS0005) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).