Gamma Tubulin Recombinant Rabbit Monoclonal Antibody

货号:
AWA10575
应用:
WB,IHC-P,IF-C,IF-T,FCM,IP
反应性:
Human,Mouse,Rat,Monkey
来源:
Rabbit
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

     

    Host Species:

    Rabbit 

    Reactivity:

    Human,Mouse,Rat,Monkey

     

    Molecular Wt:

    51 kDa


    Clonality:

    Monoclonal 

    Isotype:

    IgG

    Concentration:

    1 mg/ml


    Other Names:

    Gamma 1 tubulin; Gamma 2 tubulin; Gamma Tubulin 1; Gamma Tubulin 2; Gamma tubulin complex component 1; Gamma-2-tubulin; GCP 1; GCP1; TUBG; TUBG1; TUBG2; TUBGCP1; Tubulin; Tubulin gamma 1 chain; Tubulin gamma 2 chain; tubulin, gamma 1; tubulin, gamma 2; Xgam

     


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    -20°C,1 year



    Applications

     

    WB 1:500-1:2000

    IHC-P 1:50-1:200

    IF-C 1:50-1:200

    IF-T 1:50-1:200

    FCM 1:50-1:100

    IP Use at an assay dependent concentration.

     



    Immunogen

    Information

    Gene Name:

    TUBG1/TUBG2

    Protein Name:

    Tubulin gamma-1 chain/Tubulin gamma-2 chain


    Gene ID:

    7283/27175 (Human)     

    103733/103768 (Mouse)     

    252921/680991 (Rat)

     

    SwissPro:

    P23258/Q9NRH3 (Human)     

    P83887/Q8VCK3 (Mouse)     

    P83888/F1LUW9 (Rat)

     


    Subcellular Location:

    Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm,cytoskeleton, spindle.

     


    Immunogen:

    Recombinant protein within Human Tubulin gamma-2 chain. AA range: 227-407. 


    Specificity:

    Gamma Tubulin Monoclonal Antibody detects endogenous levels of Gamma Tubulin protein.


    Product images
    Gamma Tubulin Recombinant Rabbit Monoclonal Antibody - 1 Fig : Western blot analysis of Gamma Tubulin on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA10575, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti- Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: Hela cell
    Lane 2: Jurkat cell
    Lane 3: HEK293 cell
    Lane 4: HepG2 cell
    Lane 5: A549 cell
    Lane 6: U20S cell
    Lane 7: NIH/3T3 cell
    Lane 8: HEPA1-6 cell

    Predicted molecular weight:51KD
    Observed molecular weight:51KD

    Exposure time: 15 seconds
    Gamma Tubulin Recombinant Rabbit Monoclonal Antibody - 2 Fig : Western blot analysis of Gamma Tubulin on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA10575, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti- Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: RAW 264.7 cell
    Lane 2: Mouse brain tissue
    Lane 3: RBL-2H3 cell
    Lane 4: PC12 cell
    Lane 5: HSC-T6 cell
    Lane 6: Rat brain tissue

    Predicted molecular weight:51KD
    Observed molecular weight:51KD

    Exposure time: 90 seconds
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