SOX10 Recombinant Rabbit Monoclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA10573

应用：

WB,IHC-P,IHC-F,IF-C,IF-T,FCM,IP

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat

Molecular Wt:

50 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

DOM; SOX10; SOX 10; SRY box 10; SRY box containing gene 10; SRY related HMG box gene 10; Transcription factor SOX 10; Transcription factor SOX-10; WS2E; WS4; WS4C

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:1000-1:2000

IHC-P 1:200-1:1000

IHC-F 1:500

IF-C 1:50

IF-T 1:50

FCM 1:500-1:1000

IP 1-2μg/sample

Immunogen

Information

Gene Name：

SOX10

Protein Name：

Transcription factor SOX-10

Gene ID：

6663 (Human)

20665 (Mouse)

29361 (Rat)

SwissPro：

P56693 (Human)

Q04888 (Mouse)

O55170 (Rat)

Subcellular Location：

Cytoplasm. Nucleus. Mitochondrion outer membrane.

Immunogen：

Synthetic peptide within C terminal human SOX10.

Specificity:

SOX10 Monoclonal Antibody detects endogenous levels of SOX10 protein.

Product images
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-testis tissue with Rabbit anti-SOX10 antibody (AWA10573) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10573) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig: Fluorescence immunohistochemical analysis of Mouse-Brain tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-SOX10 antibody (AWA10573) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0688). The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10573) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (green). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.
	Fig : Western blot analysis of SOX10 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA10573, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti- Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SK-MEL-28 cell Lane 2: LN229 cell Lane 3: B16 cell Predicted molecular weight:50KD Observed molecular weight:60-75KD Exposure time: 15 seconds
	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-cerebellum tissue with Rabbit anti-SOX10 antibody (AWA10573) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10573) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.