Amyloid-β 1-42 Recombinant Rabbit Monoclonal Antibody

货号:
AWA10572
应用:
WB,IHC-P,IF
反应性:
Human,Mouse
来源:
Rabbit
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

    Host Species:

    Rabbit

    Reactivity:

    Human,Mouse

    Molecular Wt:

    4 kDa


    Clonality:

    Monoclonal

    Isotype:

    IgG

    Concentration:

    1mg/ml


    Other Names:

    A4; ABETA; ABPP; AICD-50; AICD-57; AICD-59; AID(50); AID(57); AID(59); Alzheimer disease amyloid protein; Amyloid intracellular domain 50; Amyloid intracellular domain 57; Amyloid intracellular domain 59; APP; APPI; Beta amyloid protein 42; Beta APP42; Beta-APP40;  Beta-APP42; C31; Cerebral vascular amyloid peptide; CVAP; Gamma-CTF(50); Gamma-CTF(57); Gamma-CTF(59); PN-II; PreA4; Protease nexin-II; S-APP-alpha; S-APP-beta


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.



    Applications

    WB 1:500-1:2000
    IHC-P 1:100-1:1000
    IF-C 1:50-1:200



    Immunogen

    Information

    Gene Name:

    APP

    Protein Name:

    Amyloid-beta precursor protein


    Gene ID:

    351 (Human)
    11820 (Mouse)

    SwissPro:

    P05067 (Human)
    P12023 (Mouse)


    Subcellular Location:

    Cell membrane. Membrane. Perikaryon. Cell projection, growth cone. Membrane, clathrin-coated pit. Early endosome. Cytoplasmic vesicle.


    Immunogen:

    Synthetic peptide within human Amyloid-β 1-42 peptide.


    Specificity:

    Amyloid-β 1-42 Monoclonal Antibody detects endogenous levels of Amyloid-β 1-42 protein.




    Product images
    Amyloid-β 1-42 Recombinant Rabbit Monoclonal Antibody - 1 Fig : Immunohistochemical analysis of paraffin-embedded Mouse-brain tissue with Rabbit anti-Amyloid-β 1-42 antibody (AWA10572) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10572) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
    Amyloid-β 1-42 Recombinant Rabbit Monoclonal Antibody - 2 Fig: Immunocytochemistry analysis of SH-SY5Y cells labeling Amyloid-β with Amyloid-β Recombinant Rabbit Monoclonal Antibody (AWA10572) at 1/50 dilution(green).

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Amyloid-β Recombinant Rabbit Monoclonal Antibody (AWA10572) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™488, AWS0005) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).
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