Amyloid-β 1-42 Recombinant Rabbit Monoclonal Antibody
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- 20μL
- ¥620
- 1-3个工作日
-
- 50μL
- ¥1250
- 1-3个工作日
-
- 100μL
- ¥2200
- 1-3个工作日
Product Details | Host Species: Rabbit | Reactivity: Human,Mouse | Molecular Wt: 4 kDa | |
Clonality: Monoclonal | Isotype: IgG | Concentration: 1mg/ml | ||
Other Names: A4; ABETA; ABPP; AICD-50; AICD-57; AICD-59; AID(50); AID(57); AID(59); Alzheimer disease amyloid protein; Amyloid intracellular domain 50; Amyloid intracellular domain 57; Amyloid intracellular domain 59; APP; APPI; Beta amyloid protein 42; Beta APP42; Beta-APP40; Beta-APP42; C31; Cerebral vascular amyloid peptide; CVAP; Gamma-CTF(50); Gamma-CTF(57); Gamma-CTF(59); PN-II; PreA4; Protease nexin-II; S-APP-alpha; S-APP-beta | ||||
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. | ||||
Purification: Affinity-chromatography | ||||
Storage: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage. | ||||
Applications | WB 1:500-1:2000 | |||
Immunogen Information | Gene Name: APP | Protein Name: Amyloid-beta precursor protein | ||
Gene ID: 351 (Human) | SwissPro: P05067 (Human) | |||
Subcellular Location: Cell membrane. Membrane. Perikaryon. Cell projection, growth cone. Membrane, clathrin-coated pit. Early endosome. Cytoplasmic vesicle. | ||||
Immunogen: Synthetic peptide within human Amyloid-β 1-42 peptide. | ||||
Specificity: Amyloid-β 1-42 Monoclonal Antibody detects endogenous levels of Amyloid-β 1-42 protein. | ||||
Product images | |
Fig : Immunohistochemical analysis of paraffin-embedded Mouse-brain tissue with Rabbit anti-Amyloid-β 1-42 antibody (AWA10572) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10572) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig: Immunocytochemistry analysis of SH-SY5Y cells labeling Amyloid-β with Amyloid-β Recombinant Rabbit Monoclonal Antibody (AWA10572) at 1/50 dilution(green). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Amyloid-β Recombinant Rabbit Monoclonal Antibody (AWA10572) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™488, AWS0005) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291). |
-
-
- 20μL
- ¥620
- 1-3个工作日
-
- 50μL
- ¥1250
- 1-3个工作日
-
- 100μL
- ¥2200
- 1-3个工作日
-
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