FOXP1 Recombinant Rabbit Monoclonal Antibody
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- 20μL
- ¥620
- 1-3个工作日
-
- 50μL
- ¥1250
- 1-3个工作日
-
- 100μL
- ¥2200
- 1-3个工作日
Product Details | Host Species: Rabbit | Reactivity: Human,Mouse,Rat | Molecular Wt: 77,75 kDa | |
Clonality: Monoclonal | Isotype: IgG | Concentration: 1mg/ml | ||
Other Names: FOXP1; HSPC215; Forkhead box protein P1; QRF1; 12CC4; hFKH1B; FLJ23741; MGC12942; MGC88572; MGC99551 | ||||
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. | ||||
Purification: Affinity-chromatography | ||||
Storage: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage. | ||||
Applications | WB 1:500-1:5000 | |||
Immunogen Information | Gene Name: FOXP1 | Protein Name: Forkhead box protein P1 | ||
Gene ID: 27086 (Human) | SwissPro: Q9H334 (Human) | |||
Subcellular Location: Nucleus. | ||||
Immunogen: Synthetic peptide within human FOXP1. AA range: 615-653. | ||||
Specificity: FOXP1 Monoclonal Antibody detects endogenous levels of FOXP1 protein. | ||||
Product images | |
Fig: Immunocytochemistry analysis of U-251MG cells labeling FOXP1 with Rabbit anti-FOXP1antibody (AWA10569) at 1/50 dilution(Red ). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-FOXP1 antibody (AWA10569) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, AWS0006) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291). |
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Fig : Western blot analysis of FOXP1 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA10569, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Jurkat cell Lane 2: A549 cell Lane 3: PC-3 cell Lane 4: Raji cell Lane 5: LOVO cell Lane 6: HepG2 cell Lane 7: LLC cell Lane 8: MC38 cell Lane 9: 4T1 cell Lane 10: SHZ88 cell Predicted molecular weight:75 kDa Observed molecular weight:75-100 kDa Exposure time:90 sec |
-
-
- 20μL
- ¥620
- 1-3个工作日
-
- 50μL
- ¥1250
- 1-3个工作日
-
- 100μL
- ¥2200
- 1-3个工作日
-
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