FOXP1 Recombinant Rabbit Monoclonal Antibody

货号:
AWA10569
应用:
WB,IF-C,FCM
反应性:
Human,Mouse,Rat
来源:
Rabbit
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

    Host Species:

    Rabbit

    Reactivity:

    Human,Mouse,Rat

    Molecular Wt:

    77,75 kDa


    Clonality:

    Monoclonal

    Isotype:

    IgG

    Concentration:

    1mg/ml


    Other Names:

    FOXP1; HSPC215; Forkhead box protein P1; QRF1; 12CC4; hFKH1B; FLJ23741; MGC12942; MGC88572; MGC99551


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.



    Applications

    WB 1:500-1:5000
    IF-C 1:50-1:200
    FCM 1:50-1:100



    Immunogen

    Information

    Gene Name:

    FOXP1

    Protein Name:

    Forkhead box protein P1


    Gene ID:

    27086 (Human)    
    108655 (Mouse)    
    297480 (Rat)

    SwissPro:

    Q9H334 (Human)    
    P58462 (Mouse)    
    Q498D1 (Rat)


    Subcellular Location:

    Nucleus.


    Immunogen:

    Synthetic peptide within human FOXP1. AA range: 615-653.


    Specificity:

    FOXP1 Monoclonal Antibody detects endogenous levels of FOXP1 protein.




    Product images
    FOXP1 Recombinant Rabbit Monoclonal Antibody - 1 Fig: Immunocytochemistry analysis of U-251MG cells labeling FOXP1 with Rabbit anti-FOXP1antibody (AWA10569) at 1/50 dilution(Red ).

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-FOXP1 antibody (AWA10569) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, AWS0006) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).
    FOXP1 Recombinant Rabbit Monoclonal Antibody - 2 Fig : Western blot analysis of FOXP1 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA10569, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: Jurkat cell
    Lane 2: A549 cell
    Lane 3: PC-3 cell
    Lane 4: Raji cell
    Lane 5: LOVO cell
    Lane 6: HepG2 cell
    Lane 7: LLC cell
    Lane 8: MC38 cell
    Lane 9: 4T1 cell
    Lane 10: SHZ88 cell

    Predicted molecular weight:75 kDa
    Observed molecular weight:75-100 kDa

    Exposure time:90 sec
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