Amyloid-β Recombinant Rabbit Monoclonal Antibody

货号:
AWA10568
应用:
WB,IHC-P,IHC-F,IF-T
反应性:
Human,Mouse,Rat
来源:
Rabbit
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

    Host Species:

    Rabbit

    Reactivity:

    Human,Mouse,Rat

    Molecular Wt:

    87 kDa


    Clonality:

    Monoclonal

    Isotype:

    IgG

    Concentration:

    1mg/ml


    Other Names:

    A4; ABETA; ABPP; AICD-50; AICD-57; AICD-59; AID(50); AID(57); AID(59); Alzheimer disease amyloid protein; Amyloid intracellular domain 50; Amyloid intracellular domain 57; Amyloid intracellular domain 59; APP; APPI; Beta amyloid protein 42; Beta APP42; Beta-APP40;  Beta-APP42; C31; Cerebral vascular amyloid peptide; CVAP; Gamma-CTF(50); Gamma-CTF(57); Gamma-CTF(59); PN-II; PreA4; Protease nexin-II; S-APP-alpha; S-APP-beta


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.



    Applications

    WB 1:500-1:2000
    IHC-P 1:100-1:1000
    IHC-F 1:100-1:500
    IF-T 1:100-1:500



    Immunogen

    Information

    Gene Name:

    APP

    Protein Name:

    Amyloid-beta precursor protein


    Gene ID:

    351 (Human)
    11820 (Mouse)
    54226 (Rat)

    SwissPro:

    P05067 (Human)
    P12023 (Mouse)
    P08592 (Rat)


    Subcellular Location:

    Cell membrane. Membrane. Perikaryon. Cell projection, growth cone. Membrane, clathrin-coated pit. Early endosome. Cytoplasmic vesicle.


    Immunogen:

    Synthetic peptide.


    Specificity:

    Amyloid-β Monoclonal Antibody detects endogenous levels of Amyloid-β protein.




    Product images
    Amyloid-β Recombinant Rabbit Monoclonal Antibody - 1 Fig: Fluorescence immunohistochemical analysis of Mouse-brain tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-Amyloid-β antibody (AWA10568) at 1/200 dilution.

    The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0688). The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10568) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (green). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.
    Amyloid-β Recombinant Rabbit Monoclonal Antibody - 2 Fig : Western blot analysis of Amyloid-β on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA10568, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: HeLa cell
    Lane 2: HEK-293 cell
    Lane 3: SH-SY5Y cell
    Lane 4: PC3 cell
    Lane 5: THP-1 cell
    Lane 6: A549 cell
    Lane 7: MCF-7 cell
    Lane 8: Mouse brain tissue
    Lane 9: 4T1 cell
    Lane 10: SHZ88 cell

    Predicted molecular weight:87kDa
    Observed molecular weight:110-140kDa

    Exposure time:15 sec
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