Collagen III Recombinant Rabbit Monoclonal Antibody

货号:
AWA10567
应用:
WB,FCM
反应性:
Human,Mouse,Rat
来源:
Rabbit
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

     

    Host Species:

    Rabbit

    Reactivity:

    Human,Mouse,Rat

    Molecular Wt:

    139 kDa


    Clonality:

    Monoclonal

    Isotype:

    IgG

    Concentration:

    1 mg/ml


    Other Names:

    COL3A1; Collagen alpha-1(III) chain; collagen, type III; alpha 1; EDS4A; FLJ34534


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    -20°C,1 year



    Applications

     

    WB 1:500-1:2000

    FCM 1:500-1:1000

     



    Immunogen

    Information

    Gene Name:

    COL3A1

     

    Protein Name:

    Collagen alpha-1(III) chain


    Gene ID:

    1281 (Human)

    12825 (Mouse)

    84032 (Rat)

     

    SwissPro:

    P02461 (Human)     

    P08121 (Mouse)     

    P13941 (Rat)

     


    Subcellular Location:

    Secreted, extracellular space, extracellular matrix.


    Immunogen:

    Recombinant protein within human Collagen III. AA range: 101-400.


    Specificity:

    Collagen III Monoclonal Antibody detects endogenous levels of Collagen III protein.


    Product images
    Collagen III Recombinant Rabbit Monoclonal Antibody - 1 Fig : Western blot analysis of Collagen III on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA10567, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti- Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: SH-SY5Y cell
    Lane 2: K562 cell
    Lane 3: U20S cell
    Lane 4: SIHa cell
    Lane 5: Mouse brain tissue
    Lane 6: RBL-2H3 cell
    Lane 7: PC12 cell
    Lane 8: Rat brain tissue

    Predicted molecular weight:139KD
    Observed molecular weight:150KD

    Exposure time: 45 seconds
    Collagen III Recombinant Rabbit Monoclonal Antibody - 2 Fig : Western blot analysis of Collagen III on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA10567, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti- Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: MCF-7 cell
    Lane 2: HaCaT cell

    Predicted molecular weight:139KD
    Observed molecular weight:150KD

    Exposure time:7 seconds
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